跳转至内容
Merck
CN

GE17-5268-02

Ni Sepharose™ High Performance

Cytiva 17-5268-02, pack of 100 mL

别名:

High Performance Ni Resin, Ni Sepharose

登录 查看组织和合同定价。

选择尺寸

关于此项目

NACRES:
NA.56
UNSPSC Code:
41106500

主要文件

查看所有文档
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

form

resin

crosslinking

cross-linked

packaging

pack of 100 mL

manufacturer/tradename

Cytiva 17-5268-02

storage condition

(20% ethanol)

parameter

150 cm/hr max. flow rate

technique(s)

liquid chromatography (LC): suitable

matrix

highly cross-linked 6% agarose

matrix active group

Nickel

particle size

~34 μm

cleaning in place

2-14(Ni2+ stripped medium.)

operating pH

3-12(Ni²⁺ stripped medium.)

capacity

≥40 mg binding capacity (histidine-tagged protein / mL medium)

separation technique

affinity

storage temp.

2-30°C

正在寻找类似产品? 访问 产品对比指南

相关类别

General description

Ni Sepharose™ High Performance affinity media for high-resolution, high capacity purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Ni Sepharose™ High Performance consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with Ni2+, which will selectively retain proteins with exposed histidine groups.

The high stability and broad compatibility of Ni Sepharose™ High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.

Features and Benefits

  • High-performance purification of histidine-tagged proteins
  • Negligible leakage of Ni2+ ions.
  • High binding capacity, at least 40 mg/mL medium
  • Compatible with a wide range of reducing agents, detergents, denaturants and other additives
  • 34 μm bead size for improved resolution

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
Sepharose is a trademark of Cytiva

pictograms

Environment
GHS09

signalword

Warning

hcodes

H226

存储类别

3 - Flammable liquids

法规信息

新产品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 文件 section.

如需帮助，请联系 客户支持

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

商品

Cell Lysis of Histidine-Tagged Proteins

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

Performing High-Throughput Screening Using His MultiTrap™ HP and His MultiTrap™ FF 96-Well filter Plates

This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from Cytiva.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

查看所有结果

实验方案

Purification of Protein A-Tagged Proteins

This page shows how to purify protein A-tagged proteins using Cytiva products.

Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose® High Performance

Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持