form
prepacked column
crosslinking
cross-linked
packaging
pkg of 50 ea
manufacturer/tradename
Cytiva 28-4013-53
storage condition
(0.15% Kathon CG)
parameter
600 μL sample volume
technique(s)
liquid chromatography (LC): suitable
bed volume
100 μL
matrix
highly cross-linked 6% agarose
matrix active group
Nickel
particle size
~34 μm
cleaning
2-14(Ni2+ stripped medium.)
operating pH
3-12(Ni2+ stripped medium.)
capacity
~750 μg binding capacity (histidine-tagged protein / column)
separation technique
affinity
storage temp.
2-30°C
General description
His SpinTrap™ is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC)
* See the section for licensing information in our terms and conditions.
Purification of unclarified samples on His SpinTrap™ columns minimizes loss of target protein caused by manual operations such as sample precentrifugation, transfer to centrifugation tubes, and collecting supernatant. In addition, loading unclarified sample directly to His SpinTrap™ columns reduces sample preparation time, which minimizes degradation and oxidation of sensitive target proteins.
His SpinTrap™ columns are used together with a standard microcentrifuge and one purification run takes approximately 10 min. For optimal performance, use His SpinTrap™ together with buffers prepared using His Buffer Kit.
The columns are prepacked with Ni Sepharose™ High Performance, which is precharged with nickel ions for capture of histidine-tagged proteins. Leakage of nickel ions from the Ni Sepharose™ High Performance medium is negligible.
* See the section for licensing information in our terms and conditions.
Purification of unclarified samples on His SpinTrap™ columns minimizes loss of target protein caused by manual operations such as sample precentrifugation, transfer to centrifugation tubes, and collecting supernatant. In addition, loading unclarified sample directly to His SpinTrap™ columns reduces sample preparation time, which minimizes degradation and oxidation of sensitive target proteins.
His SpinTrap™ columns are used together with a standard microcentrifuge and one purification run takes approximately 10 min. For optimal performance, use His SpinTrap™ together with buffers prepared using His Buffer Kit.
The columns are prepacked with Ni Sepharose™ High Performance, which is precharged with nickel ions for capture of histidine-tagged proteins. Leakage of nickel ions from the Ni Sepharose™ High Performance medium is negligible.
Features and Benefits
- For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.
- Ni Sepharose™ High Performance in prepacked spin columns.
- Yields up to 750 μg pure protein per column.
- Allows direct purification of both clarified and unclarified cell lysates.
Preparation Note
Storage Temp.: 4°C to 30°C
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.15% Kathon CG)
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva
signalword
Warning
hcodes
存储类别
12 - Non Combustible Liquids