His Spintrap^™

SpinTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Storage Temp.: 4°C to 30°C

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (0.15% Kathon CG)

Store at 4 to 30 °C (20% Ethanol)

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

His SpinTrap^™ is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC)

* See the section for licensing information in our terms and conditions.

Purification of unclarified samples on His SpinTrap^™ columns minimizes loss of target protein caused by manual operations such as sample precentrifugation, transfer to centrifugation tubes, and collecting supernatant. In addition, loading unclarified sample directly to His SpinTrap^™ columns reduces sample preparation time, which minimizes degradation and oxidation of sensitive target proteins.

His SpinTrap^™ columns are used together with a standard microcentrifuge and one purification run takes approximately 10 min. For optimal performance, use His SpinTrap^™ together with buffers prepared using His Buffer Kit.

The columns are prepacked with Ni Sepharose^™ High Performance, which is precharged with nickel ions for capture of histidine-tagged proteins. Leakage of nickel ions from the Ni Sepharose^™ High Performance medium is negligible.