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Merck
CN

GE28-4110-05

HiTrap® Butyl High Performance

Cytiva 28-4110-05, pack of 5 × 5 mL

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

Key Documents

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Technical Service
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ligand

butyl

packaging

pack of 5 × 5 mL

manufacturer/tradename

Cytiva 28-4110-05

parameter

<20 mL/min flow rate
42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

cross-linked agarose

particle size

24-44 μm

avg. part. size

34 μm

cleaning

2-14

working range

3-13

suitability

suitable for bioprocess medium

General description

HiTrap Butyl HP are prepacked Butyl Sepharose High Performance columns for high-resolution hydrophobic interaction chromatography (HIC). The columns are used for small-scale protein purification as well as for screening of binding and elution conditions.

Features and Benefits

  • Provide convenient and fast small-scale protein purifications.
  • Butyl Sepharose High Performance is an aliphatic HIC resin, designed for intermediate and polishing step where high resolution has priority.
  • Flexible design for use with syringes, single laboratory pump, or chromatography system such as ÄKTA design.
  • High flow rates and high capacities.
  • Excellent and easy scale-up achieved by connecting several HiTrap columns in series or using HiPrep columns.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiTrap is a registered trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Regulatory Information

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Protocols

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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