working range
3-13
ligand
butyl
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 28-4110-05
parameter
<20 mL/min flow rate, 42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
cross-linked agarose
particle size
24-44 μm
avg. part. size
34 μm
cleaning
2-14
suitability
suitable for bioprocess medium
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General description
HiTrap Butyl HP are prepacked Butyl Sepharose High Performance columns for high-resolution hydrophobic interaction chromatography (HIC). The columns are used for small-scale protein purification as well as for screening of binding and elution conditions.
Features and Benefits
- Provide convenient and fast small-scale protein purifications.
- Butyl Sepharose High Performance is an aliphatic HIC resin, designed for intermediate and polishing step where high resolution has priority.
- Flexible design for use with syringes, single laboratory pump, or chromatography system such as ÄKTA design.
- High flow rates and high capacities.
- Excellent and easy scale-up achieved by connecting several HiTrap columns in series or using HiPrep columns.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Other Notes
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Legal Information
HiTrap is a registered trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
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实验方案
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
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