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About This Item
separation technique
affinity
material
polypropylene and polyethylene plate
form
plate (prepacked)
crosslinking
cross-linked
packaging
pkg of 4 ea
manufacturer/tradename
Cytiva 28-9031-33
storage condition
(20% Ethanol)
parameter
-0.5 bar vacuum limit, 700 × g max. centrifugal force
technique(s)
liquid chromatography (LC): suitable
L × W × H
127.8 mm × 85.5 mm × 30.6 mm
bed size
50 μL , per well
well volume
800 μL
matrix
highly cross-linked 6% agarose
matrix active group
native protein A
particle size
~34 μm
operating pH
3-9
capacity
>10 mg binding capacity (human IgG / mL medium)(Human IgG)
storage temp.
2-30°C
Related Categories
General description
Protein A Sepharose™ High Performance has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen (protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). Remember to order the collection plates for washing and elution of the enriched protein.
There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.
Features and Benefits
- Fast, reliable ease-of-use format for small-scale purification of antibodies or enrichment of proteins of interest in prepacked 96-well filter plates that are ready to use.
- Flexible format, with protocols for classic elution and cross-link method.
- Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
- Fast binding kinetics and high capacity provides high yield.
- Easy scale-up with HiTrap® Protein A HP prepacked columns.
- Prepacked with Protein A Sepharose™ High Performance for coupling of antibodies of IgG subclasses.
- MultiTrap™ 96-well filter plates can be used with centrifugation or vacuum, manually or in automated robotic systems.
Preparation Note
Analysis Note
Legal Information
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
Regulatory Information
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Articles
This page describes immunoprecipitation (immunoaffinity or pull-down techniques).
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
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