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GE28-9269-73

HiScreen™ MabSelect™

Cytiva 28-9269-73

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About This Item

UNSPSC Code:
13111000
NACRES:
NA.56

Key Documents

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ligand

recombinant protein A (E. coli)

form

prepacked column

crosslinking

cross-linked

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 28-9269-73

storage condition

(20% Ehtanol)
 (20% Ethanol)

parameter

44 psi

technique(s)

liquid chromatography (LC): suitable

bed size

7.7 mm × 100 mm

bed volume

4.7 mL

column I.D.

7.7 mm

matrix

highly cross-linked agarose

matrix active group

protein A functional group

avg. part. size

85 μm (d₅₀v is the median particle size of the cumulative volume distribution)

cleaning

2-12

working range

3-10

capacity

≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)

suitability

suitable for bioprocess medium

separation technique

affinity

storage temp.

2-8°C

Related Categories

General description

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.
HiScreen™ MabSelect™ is prepacked with MabSelect™ and is part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening for capture of monoclonal antibodies (MAbs).
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Application

The small column volume of 4.7 mL and bed height of 10 cm make HiScreen™ columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up.

If necessary, two columns can easily be connected in series to give a bed height of 20 cm.

Media prepacked in HiScreen™ MabSelect™ columns are also available in other formats and as bulk packs, which makes it possible to use the same media for development work, pilot studies, and routine production.

Features and Benefits

  • MabSelect™ is a BioProcess medium for capture of MAbs from large sample volumes.
  • Prepacked, ready-to-use columns for convenient process development.
  • 10 cm bed height of HiScreen™ columns is designed to allow method optimization and parameter screening.
  • Easily connected in series to achieve 20 cm bed height.
  • Small bed volume gives fast results and minimal sample/buffer consumption.
  • Reproducible results, scalable to BioProcess columns packed with the same chromatography media using the same linear fluid velocity.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiScreen is a trademark of Cytiva
MabSelect is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Regulatory Information

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