ligand
recombinant protein A (E. coli)
表单
prepacked column
交联
cross-linked
包装
pkg of 1 ea
制造商/商品名称
Cytiva 28-9269-73
储存条件
(20% Ehtanol)
(20% Ethanol)
参数
44 psi
技术
liquid chromatography (LC): suitable
柱床尺寸
7.7 mm × 100 mm
柱床体积
4.7 mL
色谱柱内径
7.7 mm
基质
highly cross-linked agarose
基质活性基团
protein A functional group
平均零件尺寸
85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
cleaning
2-12
工作范围
3-10
容量
≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
适用性
suitable for bioprocess medium
分离技术
affinity
储存温度
2-8°C
一般描述
Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.
HiScreen™ MabSelect™ is prepacked with MabSelect™ and is part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening for capture of monoclonal antibodies (MAbs).
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
应用
The small column volume of 4.7 mL and bed height of 10 cm make HiScreen™ columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up.
If necessary, two columns can easily be connected in series to give a bed height of 20 cm.
Media prepacked in HiScreen™ MabSelect™ columns are also available in other formats and as bulk packs, which makes it possible to use the same media for development work, pilot studies, and routine production.
If necessary, two columns can easily be connected in series to give a bed height of 20 cm.
Media prepacked in HiScreen™ MabSelect™ columns are also available in other formats and as bulk packs, which makes it possible to use the same media for development work, pilot studies, and routine production.
特点和优势
- MabSelect™ is a BioProcess medium for capture of MAbs from large sample volumes.
- Prepacked, ready-to-use columns for convenient process development.
- 10 cm bed height of HiScreen™ columns is designed to allow method optimization and parameter screening.
- Easily connected in series to achieve 20 cm bed height.
- Small bed volume gives fast results and minimal sample/buffer consumption.
- Reproducible results, scalable to BioProcess columns packed with the same chromatography media using the same linear fluid velocity.
制备说明
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
HiScreen is a trademark of Cytiva
MabSelect is a trademark of Cytiva
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
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