GE28-9321-71
HIS Spintrap™ Kit
Cytiva 28-9321-71, pkg of 1 kit (50 columns + buffers)
Synonym(s):
HIS chromatography columns, HIS-tagged purification columns, IMAC columns, Ni Sepharose High Performance, immobilized metal ion columns, spin columns
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About This Item
form
prepacked column
crosslinking
cross-linked
packaging
pkg of 1 kit (50 columns + buffers)
manufacturer/tradename
Cytiva 28-9321-71
parameter
600 μL sample volume
technique(s)
liquid chromatography (LC): suitable
bed volume
100 μL
matrix
highly cross-linked 6% agarose
matrix active group
nickel
particle size
~34 μm
cleaning
2-14(Ni2+ stripped medium.)
operating pH
3-12(Ni2+ stripped medium)
capacity
~750 μg binding capacity (histidine-tagged protein / column)
separation technique
affinity
Related Categories
General description
Features and Benefits
- For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.
- Ni Sepharose High Performance in prepacked spin columns.
- Yields up to 750 µg pure protein per column.
- Allows direct purification of both clarified and unclarified cell lysates.
Preparation Note
Analysis Note
Other Notes
1 pack - GE28-4013-53 - His SpinTrap™
1 pack - GE11-0034-00 - His Buffer Kit
Legal Information
Kit Components Also Available Separately
- GE11-0034-00His Buffer Kit, Cytiva 11-0034-00SDS
- GE28-4013-53His Spintrap™, Cytiva 28-4013-53SDS
Signal Word
Danger
Hazard Statements
Storage Class Code
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
Regulatory Information
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Articles
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
How to optimize purification of histidine-tagged proteins using Cytiva products.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
Protocols
Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.
This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from Cytiva.
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