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Merck
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GE28-9321-71

HIS Spintrap™ Kit

Cytiva 28-9321-71, pkg of 1 kit (50 columns + buffers)

别名:

HIS chromatography columns, HIS-tagged purification columns, IMAC columns, Ni Sepharose High Performance, immobilized metal ion columns, spin columns

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NACRES:
NA.56
UNSPSC Code:
41106500

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产品名称

HIS Spintrap™ Kit, Cytiva 28-9321-71, pkg of 1 kit (50 columns + buffers)

form

prepacked column

crosslinking

cross-linked

packaging

pkg of 1 kit (50 columns + buffers)

manufacturer/tradename

Cytiva 28-9321-71

parameter

600 μL sample volume

technique(s)

liquid chromatography (LC): suitable

bed volume

100 μL

matrix

highly cross-linked 6% agarose

matrix active group

nickel

particle size

~34 μm

cleaning

2-14(Ni2+ stripped medium.)

operating pH

3-12(Ni2+ stripped medium)

capacity

~750 μg binding capacity (histidine-tagged protein / column)

separation technique

affinity

相关类别

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Features and Benefits

  • For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.
  • Ni Sepharose High Performance in prepacked spin columns.
  • Yields up to 750 µg pure protein per column.
  • Allows direct purification of both clarified and unclarified cell lysates.

General description

His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Other Notes

The kit contains:

1 pack - GE28-4013-53 - His SpinTrap™
1 pack - GE11-0034-00 - His Buffer Kit

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Legal Information

SpinTrap is a trademark of Cytiva

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H314

存储类别

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

法规信息

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商品

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

Optimizing Purification of Histidine-Tagged Proteins

How to optimize purification of histidine-tagged proteins using Cytiva products.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

实验方案

Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose® High Performance

Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.

Minipreps using His SpinTrap™ and His SpinTrap™ Kit

This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from Cytiva.

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