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GE28-9321-71

HIS Spintrap™ Kit

Cytiva 28-9321-71, pkg of 1 kit (50 columns + buffers)

别名:

HIS chromatography columns, HIS-tagged purification columns, IMAC columns, Ni Sepharose High Performance, immobilized metal ion columns, spin columns

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UNSPSC代码:
41106500
NACRES:
NA.56

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表单

prepacked column

交联

cross-linked

包装

pkg of 1 kit (50 columns + buffers)

制造商/商品名称

Cytiva 28-9321-71

参数

600 μL sample volume

技术

liquid chromatography (LC): suitable

柱床体积

100 μL

基质

highly cross-linked 6% agarose

基质活性基团

nickel

粒径

~34 μm

cleaning

2-14(Ni2+ stripped medium.)

工作pH值

3-12(Ni2+ stripped medium)

容量

~750 μg binding capacity (histidine-tagged protein / column)

分离技术

affinity

相关类别

一般描述

His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

特点和优势

  • For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.
  • Ni Sepharose High Performance in prepacked spin columns.
  • Yields up to 750 µg pure protein per column.
  • Allows direct purification of both clarified and unclarified cell lysates.

制备说明

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他说明

The kit contains:

1 pack - GE28-4013-53 - His SpinTrap™
1 pack - GE11-0034-00 - His Buffer Kit

法律信息

SpinTrap is a trademark of Cytiva

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • GE11-0034-00His Buffer Kit, Cytiva 11-0034-00化学品安全说明书
  • GE28-4013-53His Spintrap™, Cytiva 28-4013-53化学品安全说明书

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H314

储存分类代码

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

法规信息

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分析证书(COA)

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商品

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

Optimizing Purification of Histidine-Tagged Proteins

How to optimize purification of histidine-tagged proteins using Cytiva products.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

实验方案

Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose® High Performance

Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.

Minipreps using His SpinTrap™ and His SpinTrap™ Kit

This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from Cytiva.

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