form
prepacked column
crosslinking
cross-linked
packaging
pkg of 1 kit (50 columns + buffers)
manufacturer/tradename
Cytiva 28-9321-71
parameter
600 μL sample volume
technique(s)
liquid chromatography (LC): suitable
bed volume
100 μL
matrix
highly cross-linked 6% agarose
matrix active group
nickel
particle size
~34 μm
cleaning
2-14(Ni2+ stripped medium.)
operating pH
3-12(Ni2+ stripped medium)
capacity
~750 μg binding capacity (histidine-tagged protein / column)
separation technique
affinity
General description
His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Features and Benefits
- For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.
- Ni Sepharose High Performance in prepacked spin columns.
- Yields up to 750 µg pure protein per column.
- Allows direct purification of both clarified and unclarified cell lysates.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Other Notes
The kit contains:
1 pack - GE28-4013-53 - His SpinTrap™
1 pack - GE11-0034-00 - His Buffer Kit
1 pack - GE28-4013-53 - His SpinTrap™
1 pack - GE11-0034-00 - His Buffer Kit
Legal Information
SpinTrap is a trademark of Cytiva
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书 & 价格
- His Buffer Kit, Cytiva 11-0034-00
- His Spintrap™, Cytiva 28-4013-53
signalword
Danger
hcodes
存储类别
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects