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Merck
CN

GE28-9852-54

rProtein A GraviTrap™

Cytiva 28-9852-54, pack of 10 × 1 mL

Synonym(s):

GraviTrap

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

Key Documents

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form

prepacked column

crosslinking

cross-linked

packaging

pack of 10 × 1 mL

manufacturer/tradename

Cytiva 28-9852-54

storage condition

(20% Ethanol)

technique(s)

liquid chromatography (LC): suitable

bed volume

1 mL

matrix

(Highly cross-linked agarose, 4%)

matrix active group

Protein A functional group

particle size

90 μm
 (90 µm)

capacity

50 mg binding capacity (human IgG/column)(Protein binding capacity is protein-to-protein dependent.)

separation technique

affinity

storage temp.

2-8°C

Related Categories

General description

rProtein A GraviTrap™ includes ten ready-to-use columns prepacked with 1 mL of rProtein A Sepharose™ Fast Flow.

Application

rProtein A GraviTrap™ are prepacked gravity-fLow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on Protein A ligands in gravity-fLow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.

Features and Benefits

  • Prepacked gravity-fLow columns provide convenience, save time, and increase the reliability of your results.
  • Purification of antibodies in 30 min allows you to quickly obtain and store your target Protein in order to avoid the inherent dangers of Protein instability and degradation associated with sLow and long purification runs.
  • High purity and recovery with purity > 95% in one step and recovery typically 70% to 80%.
  • High binding capacity such as rProtein A GraviTrap™ ~ 50 mg human IgG/column

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

GraviTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Regulatory Information

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Articles

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

抗体亲和色谱的脱盐和缓冲液置换

实验室规模的脱盐是一种成熟、简单、快速的方法，可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。

Protocols

Performing a Purification of IgG Antibodies with Protein G GraviTrap™

This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service