GE28-9893-33
HiLoad® 16/600 Superdex® 75 pg
Cytiva 28-9893-33, column L × I.D. 60 cm × 16 mm, 34 μm avg. part. size
Synonym(s):
HiLoad 16/600
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About This Item
material
bed support (polyamide)
fiberglass end pieces (reinforced)
glass column
form
prepacked column
crosslinking
cross-linked
mol wt
protein Mr 10-600 kDa (fractionation range)
Mr 0.5-30 kDa (dextrans fractionation range)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 28-9893-33
storage condition
(0.2 M sodium acetate, 20% ethanol)
parameter
≤5 mL sample volume
0.3 Mpa max. pressure
0.3-1.6 mL/min flow rate (recommended)
technique(s)
liquid chromatography (LC): suitable
bed volume
120 mL
column L × I.D.
60 cm × 16 mm
matrix
cross-linked agarose, spherical
dextran-agarose particle platform
matrix active group
agarose
dextran-agarose phase
particle size
24-44 μm
avg. part. size
34 μm
cleaning
1-14
working range
3-12
separation technique
size exclusion (SEC)
shipped in
ambient
storage temp.
2-30°C
Related Categories
General description
Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.
Application
Features and Benefits
- High resolution with short run times and good recovery.
- High capacity at high flow rates.
- Separations can be scaled up to production levels.
- Prepacked for convenience and reproducibility.
- 1/16” fittings for convenient connections to AKTA design systems.
Preparation Note
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
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