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Merck
CN

GE28-9893-33

Sigma-Aldrich

HiLoad® 16/600 Superdex® 75 pg

Cytiva 28-9893-33, column L × I.D. 60 cm × 16 mm, 34 μm avg. part. size

别名:

HiLoad 16/600

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UNSPSC代码:
41115700
NACRES:
SB.52

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物料

bed support (polyamide)
fiberglass end pieces (reinforced)
glass column

表单

prepacked column

交联

cross-linked

分子量

protein Mr 10-600 kDa (fractionation range)
Mr 0.5-30 kDa (dextrans fractionation range)

包装

pkg of 1 ea

制造商/商品名称

Cytiva 28-9893-33

储存条件

(0.2 M sodium acetate, 20% ethanol)

参数

≤5 mL sample volume
0.3 Mpa max. pressure
0.3-1.6 mL/min flow rate (recommended)

技术

liquid chromatography (LC): suitable

柱床体积

120 mL

色谱柱长度 × 内径

60 cm × 16 mm

基质

cross-linked agarose, spherical
 dextran-agarose particle platform

基质活性基团

agarose
dextran-agarose phase

粒径

24-44 μm

平均零件尺寸

34 μm

cleaning

1-14

工作范围

3-12

分离技术

size exclusion (SEC)

运输

ambient

储存温度

2-30°C

相关类别

一般描述

HiLoad® 16/600 Superdex® 75 prep grade are prepacked 120 mL XK columns designed for high-resolution preparative gel filtration chromatography separating proteins and polynucleotides.

Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.

应用

All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile
for high-resolution preparative gel filtration chromatography separating proteins and polynucleotides

特点和优势

  • High resolution with short run times and good recovery.
  • High capacity at high flow rates.
  • Separations can be scaled up to production levels.
  • Prepacked for convenience and reproducibility.
  • 1/16” fittings for convenient connections to AKTA design systems.

制备说明

Storage Temp.: 4°C to 30°C
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他说明

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

法律信息

HiLoad is a registered trademark of Cytiva
Superdex is a registered trademark of Cytiva

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

Performing an Isolation of Recombinant Protein Complexes

This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from Cytiva.

Setting Pressure Limits for Size Exclusion Chromatography

Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.

实验方案

使用Superdex进行分离

Cytiva的Superdex是由葡聚糖和琼脂糖的复合基础基质组成的SEC介质。本页面介绍如何使用superdex执行分离任务。

Performing a Separation with Superdex

Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

Refolding of proteins using gel filtration

This page shows how to refold proteins using gel filtration and products from Cytiva.

Purification from Unclarified Cell Lysate using HisTrap™ FF Crude

This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from Cytiva.

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相关内容

Separation Options with Superdex®

Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.

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