物料
bed support (polyamide)
glass column
glass fiber (reinforced)
分子量
3000-70000 Da (globular proteins)
包装
pkg of 1 ea
制造商/商品名称
Cytiva 28-9893-33
参数
<1.0 mL/min flow rate
<5 mL sample volume
0.3 Mpa (42 psi)
柱床体积
120 mL
色谱柱长度 × 内径
60 cm × 16 mm
基质
agarose-dextran composite
dextran-agarose particle platform
polymeric
基质活性基团
dextran-agarose phase
dextran-agarose phase
平均零件尺寸
34 μm
cleaning
1-14
工作范围
3-12
分离技术
size exclusion (SEC)
一般描述
HiLoad® 16/600 Superdex® 75 prep grade are prepacked 120 mL XK columns designed for high-resolution preparative gel filtration chromatography separating proteins and polynucleotides.
Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.
Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.
应用
All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile
for high-resolution preparative gel filtration chromatography separating proteins and polynucleotides
特点和优势
- High resolution with short run times and good recovery.
- High capacity at high flow rates.
- Separations can be scaled up to production levels.
- Prepacked for convenience and reproducibility.
- 1/16” fittings for convenient connections to AKTA design systems.
制备说明
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
HiLoad is a registered trademark of Cytiva
Superdex is a registered trademark of Cytiva
警示用语:
Warning
危险声明
储存分类代码
3 - Flammable liquids
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