material
bed support (polyamide), fiberglass end pieces (reinforced), glass column
form
prepacked column
crosslinking
cross-linked
mol wt
protein Mr 10-600 kDa (fractionation range), Mr 0.5-30 kDa (dextrans fractionation range)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 28-9893-33
storage condition
(0.2 M sodium acetate, 20% ethanol)
parameter
≤5 mL sample volume, 0.3 Mpa max. pressure, 0.3-1.6 mL/min flow rate (recommended)
technique(s)
liquid chromatography (LC): suitable
bed volume
120 mL
column L × I.D.
60 cm × 16 mm
matrix
cross-linked agarose, spherical, dextran-agarose particle platform
matrix active group
agarose, dextran-agarose phase
particle size
24-44 μm
avg. part. size
34 μm
cleaning
1-14
working range
3-12
separation technique
size exclusion (SEC)
shipped in
ambient
storage temp.
2-30°C
相关类别
General description
Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.
Application
Features and Benefits
- High resolution with short run times and good recovery.
- High capacity at high flow rates.
- Separations can be scaled up to production levels.
- Prepacked for convenience and reproducibility.
- 1/16” fittings for convenient connections to AKTA design systems.
Preparation Note
Analysis Note
Legal Information
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
商品
This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from Cytiva.
Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.
实验方案
Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.
This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from Cytiva.
This page shows how to refold proteins using gel filtration and products from Cytiva.
Ensure chromatographic sample clarity to avoid column clogging, reduce washing needs, and prolong column life for purification.
相关内容
Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.
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