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GE28-9893-35

HiLoad® 16/600 Superdex® 200 pg

Cytiva 28-9893-35, column L × I.D. 60 cm × 16 mm, 34 μm avg. part. size

Synonym(s):

16/600 Superdex 200, HiLoad Superdex 200

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About This Item

NACRES:
SB.52
UNSPSC Code:
41115700
Technical Service
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material

bed support (polyamide), fiberglass end pieces (polypropylene reinforced), glass column

form

prepacked column

crosslinking

cross-linked

mol wt

protein Mr 10-600 kDa (fractionation range), Mr 1-100 kDa (dextrans fractionation range)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 28-9893-35

storage condition

(20% Ethanol )

parameter

<5 mL sample volume, 0.3 Mpa max. pressure, 0.3-1.6 mL/min flow rate (recommended)

technique(s)

liquid chromatography (LC): suitable

bed volume

120 mL

column L × I.D.

60 cm × 16 mm

matrix

cross-linked agaorse, spherical, dextran-agarose particle platform

matrix active group

agarose, dextran-agarose phase

avg. part. size

34 μm

cleaning

1-14

working range

3-12

separation technique

size exclusion (SEC)

shipped in

ambient

storage temp.

2-30°C

General description

HiLoad® 16/600 Superdex® 200 prep grade are prepacked XK columns designed for high-resolution preparative gel filtration chromatography. The column is an excellent choice for separating MAbs from dimers and contaminants.

Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.

Application

All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile
for high-resolution preparative gel filtration chromatography, e.g, separate mAbs from dimers and contaminants

Features and Benefits

  • High resolution with short run times and good recovery.
  • High capacity at high flow rates.
  • Separations can be scaled up to production levels.
  • Prepacked for convenience and reproducibility.
  • 1/16” fittings for convenient connections to AKTA design systems.

Preparation Note

Storage Temp.: 4°C to 30°C
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

HiLoad is a registered trademark of Cytiva
Superdex is a registered trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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