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GE28-9893-35

HiLoad® 16/600 Superdex® 200 pg

Cytiva 28-9893-35, column L × I.D. 60 cm × 16 mm, 34 μm avg. part. size

Synonym(s):

16/600 Superdex 200, HiLoad Superdex 200

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About This Item

NACRES:
SB.52
UNSPSC Code:
41115700
Technical Service
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material

bed support (polyamide), fiberglass end pieces (polypropylene reinforced), glass column

form

prepacked column

crosslinking

cross-linked

mol wt

protein Mr 10-600 kDa (fractionation range), Mr 1-100 kDa (dextrans fractionation range)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 28-9893-35

storage condition

(20% Ethanol )

parameter

<5 mL sample volume, 0.3 Mpa max. pressure, 0.3-1.6 mL/min flow rate (recommended)

technique(s)

liquid chromatography (LC): suitable

bed volume

120 mL

column L × I.D.

60 cm × 16 mm

matrix

cross-linked agaorse, spherical, dextran-agarose particle platform

matrix active group

agarose, dextran-agarose phase

avg. part. size

34 μm

cleaning

1-14

working range

3-12

separation technique

size exclusion (SEC)

shipped in

ambient

storage temp.

2-30°C

General description

HiLoad® 16/600 Superdex® 200 prep grade are prepacked XK columns designed for high-resolution preparative gel filtration chromatography. The column is an excellent choice for separating MAbs from dimers and contaminants.

Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.

Application

All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile
for high-resolution preparative gel filtration chromatography, e.g, separate mAbs from dimers and contaminants

Features and Benefits

  • High resolution with short run times and good recovery.
  • High capacity at high flow rates.
  • Separations can be scaled up to production levels.
  • Prepacked for convenience and reproducibility.
  • 1/16” fittings for convenient connections to AKTA design systems.

Preparation Note

Storage Temp.: 4°C to 30°C
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

HiLoad is a registered trademark of Cytiva
Superdex is a registered trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Protocols

Performing a Separation with Superdex

Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

Two-step Purification of Mouse Monoclonal IgG1 Using HiTrap® Protein G HP for the Capture Step

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.

Refolding of proteins using gel filtration

This page shows how to refold proteins using gel filtration and products from Cytiva.

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Articles

Optimizing Purification of Histidine-Tagged Proteins

How to optimize purification of histidine-tagged proteins using Cytiva products.

Performing an Isolation of Recombinant Protein Complexes

This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from Cytiva.

Setting Pressure Limits for Size Exclusion Chromatography

Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.

Related Content

Separation Options with Superdex®

Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.

Global Trade Item Number

SKUGTIN
GE28-9893-3504061838551696