GE28-9909-45
Superdex® 200 Increase 5/150 GL
Cytiva 28-9909-45, column L × I.D. 15 cm × 5 mm, 8.6 μm particle size
Synonym(s):
Size-exclusion chromatography column
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About This Item
material
glass column
polyethylene filter
product line
Superdex®
form
prepacked column
crosslinking
cross-linked
mol wt
protein Mr 10-600 kDa (fractionation range)
Mp 1-100 kDa (dextrans fractionation range)
Mr 1.3 MDa (exclusion limit)
manufacturer/tradename
Cytiva 28-9909-45
packaging
1 ea of
storage condition
(20% ethanol )
parameter
0.45 mL/min flow rate
0.75 mL/min max. flow rate
3.0 Mpa max. pressure (725 psi)
4-50 μL sample volume
technique(s)
liquid chromatography (LC): suitable
bed volume
~3 mL
column L × I.D.
15 cm × 5 mm
matrix
agarose dextran composite
matrix active group
agarose and dextrans composite
particle size
8.6 μm
cleaning
1-14
working range
3-12
separation technique
size exclusion (SEC)
shipped in
ambient
storage temp.
2-30°C
Related Categories
General description
Superdex™ 200 Increase is a next-generation, dextran-agarose composite matrix for SEC. With smaller and more rigid beads than their predecessors, Superdex™ Increase columns deliver higher resolution purification in shorter run times.
Superdex™ 200 Increase has a selectivity curve optimized for excellent resolution of antibodies and macromolecules within the range Mr ~ 100 000 to ~ 300 000. It is well suited for detection and separation of antibody monomers, dimers, and aggregates present in monoclonal antibody preparations. The outstanding resolving power of this resin facilitates protein characterization, including analysis of antibody size variants, assessment of membrane protein size homogeneity, and the study of protein-protein interactions.
Superdex™ 200 Increase is optimized to give high resolution separation for proteins with molecular weights in the range Mr ~ 100 000 to ~ 300 000. Superose™ 6 Increase provides a complementary fractionation range, resolving very large protein complexes and macromolecules that Superdex 200 Increase cannot separate.
This agarose-based resin is alkali-resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different proteins, with minimal risk of carry-over between samples.
Application
Features and Benefits
- Enhanced performance: improved resolution and runtime compared to predecessor Superdex™ 200.
- Very high resolution: small bead size and narrow particle size distribution provide high resolution, for high protein purity.
- High flow rates: rigid beads give excellent pressure/flow properties.
Preparation Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
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Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.
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