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Merck
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GE29-0485-65

HiTrap® TALON® Crude

Cytiva 29-0485-65, pack of 1 mL

Synonym(s):

HIS chromatography columns, HIS columns, HIS-tagged purification columns, IMAC columns, TALON Superflow cobalt-IMAC, immobilized metal ion chromatography columns

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About This Item

NACRES:
NA.56
UNSPSC Code:
23151816

Key Documents

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separation technique

affinity

storage temp.

2-8°C

form

prepacked column

crosslinking

cross-linked

packaging

pack of 1 mL

manufacturer/tradename

Cytiva 29-0485-65

storage condition

(20% ethanol)

parameter

0.5 Mpa max. pressure, 1 mL/min flow rate, 4 mL/min max. flow rate

technique(s)

liquid chromatography (LC): suitable

bed volume

1 mL

column I.D. × L

7 mm × 25 mm

matrix

6% cross-linked agarose

matrix active group

cobalt

particle size

60-160 μm

cleaning

2-14(Co2+ stripped medium)

operating pH

3-12(Co2+ stripped medium)

capacity

≤20 mg binding capacity (histidine-tagged protein)

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General description

HiTrap TALON crude 1 ml and 5 ml columns allow for rapid separations and easy scale-up with a minimum of sample preparation and equipment. TALON Superflow™ is a cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. TALON Superflow and prepacked HiTrap TALON crude columns allow protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Application

HiTrap TALON crude columns allow for rapid separations and easy scale-up. Samples can be applied directly to the column after thorough cell disruption - without precentrifugation and filtration. The filters in the top and bottom of the column make it possible to directly load unclarified lysates on the column without causing back pressure problems or leakage of the TALON Superflow beads. Prior to loading samples on HiTrap TALON crude columns, cell lysate should be prepared using methods such as sonication, homogenization, or freeze/thaw.

Features and Benefits

  • Columns prepacked with cobalt-based chromatography media designed for purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC).
  • Suitable for IMAC purification of histidine-tagged proteins when Ni2+ is not the optimal choice of metal ion.
  • Compatible with commonly used IMAC reagents and appropriate for purifying proteins under native or denaturing conditions.
  • Prepacked HiTrap TALON crude columns save time by allowing the application of unclarified samples directly, minimizing labor and degradation of proteins.
  • HiTrap TALON crude columns allow repeated runs without stripping, cleaning, or Co2+ recharging.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiTrap is a registered trademark of Cytiva
Superflow is a trademark of Sterogene Bioseparations Inc.
Talon is a registered trademark of Clontech

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Regulatory Information

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Articles

Cell Lysis of Histidine-Tagged Proteins

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

Manual and Automated Purification

This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

Protocols

Purification of Histidine-Tagged Proteins using TALON® Superflow

This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Handling Inclusion Bodies in Recombinant Protein Expression

How to isolate proteins from inclusion bodies using Cytiva products.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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