form
prepacked column
crosslinking
cross-linked
packaging
pack of 1 mL
manufacturer/tradename
Cytiva 29-0485-65
storage condition
(20% ethanol)
parameter
0.5 Mpa max. pressure, 1 mL/min flow rate, 4 mL/min max. flow rate
technique(s)
liquid chromatography (LC): suitable
bed volume
1 mL
column I.D. × L
7 mm × 25 mm
matrix
6% cross-linked agarose
matrix active group
cobalt
particle size
60-160 μm
cleaning
2-14(Co2+ stripped medium)
operating pH
3-12(Co2+ stripped medium)
capacity
≤20 mg binding capacity (histidine-tagged protein)
separation technique
affinity
storage temp.
2-8°C
General description
HiTrap TALON crude 1 ml and 5 ml columns allow for rapid separations and easy scale-up with a minimum of sample preparation and equipment. TALON Superflow™ is a cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. TALON Superflow and prepacked HiTrap TALON crude columns allow protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Application
HiTrap TALON crude columns allow for rapid separations and easy scale-up. Samples can be applied directly to the column after thorough cell disruption - without precentrifugation and filtration. The filters in the top and bottom of the column make it possible to directly load unclarified lysates on the column without causing back pressure problems or leakage of the TALON Superflow beads. Prior to loading samples on HiTrap TALON crude columns, cell lysate should be prepared using methods such as sonication, homogenization, or freeze/thaw.
Features and Benefits
- Columns prepacked with cobalt-based chromatography media designed for purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC).
- Suitable for IMAC purification of histidine-tagged proteins when Ni2+ is not the optimal choice of metal ion.
- Compatible with commonly used IMAC reagents and appropriate for purifying proteins under native or denaturing conditions.
- Prepacked HiTrap TALON crude columns save time by allowing the application of unclarified samples directly, minimizing labor and degradation of proteins.
- HiTrap TALON crude columns allow repeated runs without stripping, cleaning, or Co2+ recharging.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Other Notes
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Legal Information
HiTrap is a registered trademark of Cytiva
Superflow is a trademark of Sterogene Bioseparations Inc.
Talon is a registered trademark of Clontech
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Warning
hcodes
存储类别
3 - Flammable liquids