GE29-0491-04
HiTrap® MabSelect SuRe™
Cytiva 29-0491-04, pack of 1 mL
Synonym(s):
affinity column, chromatography column, mAb purification column, prepacked column, protein A column
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About This Item
form
prepacked column
crosslinking
cross-linked
packaging
pack of 1 mL
manufacturer/tradename
Cytiva 29-0491-04
storage condition
(20% Ethanol)
parameter
0.5 mL/min flow rate (recommended)
4 mL/min max. flow rate
5 bar max. pressure
technique(s)
liquid chromatography (LC): suitable
bed volume
1 mL
column I.D. × L
7 mm × 25 mm
matrix
highly cross-linked agarose
matrix active group
alkali-stabilized protein A-derived (E. coli)
avg. part. size
~85 μm
cleaning
3-13.7
working range
3-12
capacity
~35 mg binding capacity (human lgG / ml resin)
separation technique
affinity
storage temp.
2-8°C
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General description
Features and Benefits
- Withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
- Novel ligand design gives enhanced protease resistance resulting in lower ligand leakage.
- Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
- Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
- Prepacked HiTrap columns for process development, screening of purification conditions, and small-scale purification of Mabs.
Preparation Note
Other Notes
Legal Information
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a Cytiva company and its licensed distributors. Any other use will require a separate license from a Cytiva company.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Regulatory Information
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Articles
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
实验室规模的脱盐是一种成熟、简单、快速的方法，可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。
Protocols
This page shows how to separate IgG antibodies by affinity chromatography using MabSelect Sure from Cytiva.
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