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Merck
CN

GE29-0491-04

HiTrap® MabSelect SuRe™

Cytiva 29-0491-04, pack of 1 mL

别名:

affinity column, chromatography column, mAb purification column, prepacked column, protein A column

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NACRES:
NA.56
UNSPSC Code:
23151816

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form

prepacked column

crosslinking

cross-linked

packaging

pack of 1 mL

manufacturer/tradename

Cytiva 29-0491-04

storage condition

(20% Ethanol)

parameter

0.5 mL/min flow rate (recommended), 4 mL/min max. flow rate, 5 bar max. pressure

technique(s)

liquid chromatography (LC): suitable

bed volume

1 mL

column I.D. × L

7 mm × 25 mm

matrix

highly cross-linked agarose

matrix active group

alkali-stabilized protein A-derived (E. coli)

avg. part. size

~85 μm

cleaning

3-13.7

working range

3-12

capacity

~35 mg binding capacity (human lgG / ml resin)

separation technique

affinity

storage temp.

2-8°C

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General description

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.
HiTrap MabSelect SuRe columns are prepacked with MabSelect SuRe, a BioProcess medium for purification of monoclonal antibodies from large sample volumes. The novel alkali-tolerant recombinant Protein A ligand allows cleaning-in-place (CIP).

Features and Benefits

  • Withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
  • Novel ligand design gives enhanced protease resistance resulting in lower ligand leakage.
  • Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
  • Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
  • Prepacked HiTrap columns for process development, screening of purification conditions, and small-scale purification of Mabs.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

Free MabSelect SuRe Ligand
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a Cytiva company and its licensed distributors. Any other use will require a separate license from a Cytiva company.
HiTrap is a registered trademark of Cytiva
MabSelect SuRe is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

存储类别

3 - Flammable liquids

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商品

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

抗体亲和色谱的脱盐和缓冲液置换

实验室规模的脱盐是一种成熟、简单、快速的方法，可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。

实验方案

Performing a Purification of IgG Antibodies with MabSelect Sure

This page shows how to separate IgG antibodies by affinity chromatography using MabSelect Sure from Cytiva.

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