GE29-0510-21
HisTrap™ High Performance
Cytiva 29-0510-21, pack of 1 mL
Synonym(s):
HIS chromatography column, HIS-tagged purification columns, IMAC column, Ni Sepharose HP, immobilized metal ion chromatography column, prepacked column
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About This Item
form
prepacked column
crosslinking
cross-linked
packaging
pack of 1 mL
manufacturer/tradename
Cytiva 29-0510-21
storage condition
(20% ethanol)
parameter
5 bar max. pressure
5 mL/min flow rate (recommended)
technique(s)
liquid chromatography (LC): suitable
bed volume
1 mL
column I.D. × L
7 mm × 25 mm
matrix
highly cross-linked 6% agarose
matrix active group
Ni2+
avg. part. size
34 μm
cleaning
2-14(Ni2+-stripped medium.)
working range
3-12(Ni2+-stripped medium)
capacity
≥40 mg binding capacity (histidine-tagged protein / ml medium)
separation technique
affinity
shipped in
ambient
storage temp.
2-30°C
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General description
Application
Features and Benefits
- High binding capacity, at least 40 mg/ml chromatography medium.
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
- Negligible Ni2+ leakage
- Simple manual operation with a syringe, pump, or chromatography system such as AKTA design
Preparation Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Regulatory Information
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Articles
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from Cytiva.
This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap™ FF columns and ÄKTAprime from Cytiva.
Protocols
This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.
This page shows how to condition membrane proteins for further analysis with products from Cytiva.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
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