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Merck
CN

GE29-0510-21

HisTrap™ High Performance

Cytiva 29-0510-21, pack of 1 mL

别名:

HIS chromatography column, HIS-tagged purification columns, IMAC column, Ni Sepharose HP, immobilized metal ion chromatography column, prepacked column

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UNSPSC代码:
23151816
NACRES:
NA.56

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表单

prepacked column

交联

cross-linked

包装

pack of 1 mL

制造商/商品名称

Cytiva 29-0510-21

储存条件

(20% ethanol)

参数

5 bar max. pressure
5 mL/min flow rate (recommended)

技术

liquid chromatography (LC): suitable

柱床体积

1 mL

色谱柱内径 × 长度

7 mm × 25 mm

基质

highly cross-linked 6% agarose

基质活性基团

Ni2+

平均零件尺寸

34 μm

cleaning

2-14(Ni2+-stripped medium.)

工作范围

3-12(Ni2+-stripped medium)

容量

≥40 mg binding capacity (histidine-tagged protein / ml medium)

分离技术

affinity

运输

ambient

储存温度

2-30°C

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一般描述

HisTrap HP columns are prepacked with Ni Sepharose High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

应用

HisTrap HP columns are designed for simple, one-step purification of histidine-tagged proteins. The columns are prepacked with Ni Sepharose High Performance, which has high binding capacity and low nickel ion leakage that ensures reliable capture of target protein in repeated IMAC purifications.

特点和优势

  • High binding capacity, at least 40 mg/ml chromatography medium.
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
  • Negligible Ni2+ leakage
  • Simple manual operation with a syringe, pump, or chromatography system such as AKTA design

制备说明

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

法律信息

HisTrap is a trademark of Cytiva

象形图

Environment
GHS09

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

法规信息

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商品

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Performing an Isolation of Recombinant Protein Complexes

This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from Cytiva.

Performing a Purification and On-column Refolding of an Insoluble Histidine-tagged Protein

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap™ FF columns and ÄKTAprime from Cytiva.

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实验方案

Sepharose High Performance: Purification with High Resolution

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from Cytiva.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

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