GE29-0915-98
Superose® 6 Increase
3.2/300, Cytiva 29-0915-98, 8.6 μm avg. part. size
Synonym(s):
Size exclusion chromatography, Superose 6 column
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About This Item
material
glass column
product line
Superose®
form
prepacked column
crosslinking
cross-linked
mol wt
protein Mr 40 MDa (exclusion limit)
protein Mr 5-5000 kDa (fractionation range)
Mp 1-300 kDa (dextrans fractionation range)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 29-0915-98
storage condition
(20% ethanol )
parameter
0.04 mL/min flow rate (recommended)
0.15 mL/min max. flow rate
2 Mpa pressure drop required
4-50 μL sample volume
technique(s)
liquid chromatography (LC): suitable
bed diam. × H
3.2 mm × 300 mm
bed volume
2.4 mL
matrix
cross-linked agarose
matrix active group
agarose
avg. part. size
8.6 μm
cleaning
1-14
working range
3-12
separation technique
size exclusion (SEC)
shipped in
ambient
storage temp.
2-30°C
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General description
Application
Features and Benefits
- Increased resolution compared with Superose 6, for higher purity and improved analysis results.
- Reduced runtime compared with Superose 6, for rapid results.
- Versatile use for both preparative and analytical purposes, especially for large proteins and complexes.
- Tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over.
Preparation Note
Analysis Note
Legal Information
Storage Class Code
3 - Flammable liquids
Regulatory Information
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Articles
Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.
Protocols
Superose from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superose column.
Ensure chromatographic sample clarity to avoid column clogging, reduce washing needs, and prolong column life for purification.
Related Content
Separation options for Size Exclusion Chromatography with Superose from Cytiva.
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