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Merck
CN

GE29-1487-22

Sigma-Aldrich

Superdex® Increase 75

5/150 GL, Cytiva 29-1487-22, column L × I.D. 15 cm × 5 mm, 9 μm avg. part. size

Synonym(s):

Superdex 75 Column, Superdex Increase

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About This Item

UNSPSC Code:
41115711
NACRES:
SB.52

Key Documents

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material

glass column
titanium frit

product line

Superdex®

form

prepacked column

crosslinking

cross-linked

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

mol wt

protein Mr 100 kDa (exclusion limit)
protein Mr 3-70 kDa (fractionation range)
 3000-70,000 Da (globular proteins)
Mp 0.5-30 kDa (dextrans fractionation range)

packaging

pkg of 1 ea
1 ea of

manufacturer/tradename

Cytiva 29-1487-22

storage condition

( 20% ethanol )

parameter

≤0.75 mL/min max. flow rate
0.45 mL/min flow rate (recommended)
3000 kPa pressure drop required
4-50 μL sample volume

technique(s)

liquid chromatography (LC): suitable

bed volume

~3 mL

column L × I.D.

15 cm × 5 mm

matrix

cross-linked agarose and dextran

matrix active group

dextran-agarose

avg. part. size

9 μm

cleaning in place

1-14

operating pH range

3-12

working range

3-12

separation technique

size exclusion (SEC)

shipped in

ambient

storage temp.

2-30°C

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General description

Our new generation columns called "Increase" offer higher resolution and shorter run times than their predecessors. Superdex 75 Increase is designed for small-scale preparative purification and analysis of recombinant tagged or other proteins with molecular weights from 3000 up to 70 000, such as recombinant tagged proteins

Features and Benefits

Comprising smaller, more rigid beads with a narrower particle size distribution and a higher selectivity, Superdex 75 Increase columns offer:
  • Up to 50% higher resolution compared with predecessor Superdex 75, for improved purity and analysis results.
  • Runtime reduced down to one third compared with predecessor Superdex 75, with maintained resolution for results faster.
  • Versatile use in both preparative and analytical applications, especially with recombinant tagged proteins.
  • Tolerance to repeated harsh cleaning procedures at high pH ensures long column lifetime and minimizes carryover.
  • Higher lot-to-lot consistency compared with predecessor Superdex 75.

Preparation Note

Storage Temp.: 4°C to 30°C
Store at 4-30 °C (20% ethanol)

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Superdex is a registered trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

104.9 °F

Flash Point(C)

40.5 °C

Regulatory Information

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