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Merck
CN

GE29-1487-22

Sigma-Aldrich

Superdex® Increase 75

5/150 GL, Cytiva 29-1487-22, column L × I.D. 15 cm × 5 mm, 9 μm avg. part. size

别名:

Superdex 75 Column, Superdex Increase

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UNSPSC代码:
41115711
NACRES:
SB.52

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物料

glass column
titanium frit

产品线

Superdex®

表单

prepacked column

交联

cross-linked

保质期

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分子量

protein Mr 100 kDa (exclusion limit)
protein Mr 3-70 kDa (fractionation range)
 3000-70,000 Da (globular proteins)
Mp 0.5-30 kDa (dextrans fractionation range)

包装

pkg of 1 ea
1 ea of

制造商/商品名称

Cytiva 29-1487-22

储存条件

( 20% ethanol )

参数

≤0.75 mL/min max. flow rate
0.45 mL/min flow rate (recommended)
3000 kPa pressure drop required
4-50 μL sample volume

技术

liquid chromatography (LC): suitable

柱床体积

~3 mL

色谱柱长度 × 内径

15 cm × 5 mm

基质

cross-linked agarose and dextran

基质活性基团

dextran-agarose

平均零件尺寸

9 μm

cleaning in place

1-14

工作pH范围

3-12

工作范围

3-12

分离技术

size exclusion (SEC)

运输

ambient

储存温度

2-30°C

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相关类别

一般描述

Our new generation columns called "Increase" offer higher resolution and shorter run times than their predecessors. Superdex 75 Increase is designed for small-scale preparative purification and analysis of recombinant tagged or other proteins with molecular weights from 3000 up to 70 000, such as recombinant tagged proteins

特点和优势

Comprising smaller, more rigid beads with a narrower particle size distribution and a higher selectivity, Superdex 75 Increase columns offer:
  • Up to 50% higher resolution compared with predecessor Superdex 75, for improved purity and analysis results.
  • Runtime reduced down to one third compared with predecessor Superdex 75, with maintained resolution for results faster.
  • Versatile use in both preparative and analytical applications, especially with recombinant tagged proteins.
  • Tolerance to repeated harsh cleaning procedures at high pH ensures long column lifetime and minimizes carryover.
  • Higher lot-to-lot consistency compared with predecessor Superdex 75.

制备说明

Storage Temp.: 4°C to 30°C
Store at 4-30 °C (20% ethanol)

其他说明

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

法律信息

Superdex is a registered trademark of Cytiva

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

危险分类

Flam. Liq. 3

储存分类代码

3 - Flammable liquids

WGK

WGK 1

闪点(°F)

104.9 °F

闪点(°C)

40.5 °C

法规信息

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分析证书(COA)

Lot/Batch Number

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