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Merck
CN

GE29323952

Superose® 6 Prep Grade HiLoad®

column I.D. × L 16 mm × 600 mm, Cytiva 29323952

Synonym(s):

Superose 6 Column

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About This Item

UNSPSC Code:
41115709
NACRES:
NB.21
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form

prepacked column

crosslinking

cross-linked

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 29323952

storage condition

(20% ethanol)

parameter

flow rate (1.6 mL/min)
≤5 mL sample volume
0.3 Mpa max. pressure
1.6 mL/min max. flow rate

technique(s)

liquid chromatography (LC): suitable

bed volume

120 mL

column I.D. × L

16 mm × 600 mm

matrix

cross-linked agarose

matrix active group

agarose

particle size

~30 μm

pore size

40000 kDa exclusion limit
5-5000 kDa fractionation range (globular proteins)

cleaning in place

1-14

operating pH

3—12

separation technique

size exclusion (SEC)

storage temp.

2-30°C

Related Categories

General description

HiLoad 16/600 Superose 6 prep grade (pg) is a prepacked size exclusion chromatography (SEC) column for high-resolution purification of large biomolecules and protein complexes. The column is an excellent choice for purification of sample volumes up to 5 mL.


Selectivity for a wide range of proteins: This column is packed with Superose 6 pg resin, which provides good resolution separations of proteins and other biomolecules according to size. The wide fractionation range of the resin enables reproducible and robust separations in the molecular weight range from 5000 up to 5 000 000.

Features and Benefits

  • Convenience and reproducibility: can be used with standalone pump or a chromatography system.
  • High-resolution separations: purification is possible across an exceptionally broad molecular-weight range.
  • Scalable – separations can be scaled up using Superose 6 prep grade resin.
  • ÄKTA system compatible – Fittings (1/16”) for direct connection to ÄKTA systems and other chromatography systems.

Preparation Note

Storage Temp.: 4°C to 30°C

Legal Information

HiLoad is a registered trademark of Cytiva
Superose is a registered trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

96.8 °F

Flash Point(C)

36 °C

Regulatory Information

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