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About This Item
NACRES:
NA.53
UNSPSC Code:
12352204
Biological source:
Escherichia coli
biological source
Escherichia coli
packaging
pack of 5000 units
manufacturer/tradename
Cytiva E70073X
shipped in
dry ice
storage temp.
−20°C
General description
Exonuclease I (Exo I) from E. coli is an Mg2+ ion dependent exonuclease.
Acts specifically on single-stranded DNA degrading it processively in the 3′- to 5′-direction, producing 5′-mononucleotides.
Application
Exonuclease I has been used to quantify the expression of 56 genes in each F2 XY gonad.
Biochem/physiol Actions
Exonuclease I (Exo I) from E. coli can degrade single-stranded DNA (ssDNA) from 3′ to 5′. It participates in various DNA repair systems, like, RecBCD-dependent homologous recombination, methyl-dependent mismatch repair and genomic tandem repeat maintenance.
Features and Benefits
- Elimination of residual single-stranded DNA containing a 3′-terminus.
- Measuring endonucleolytic cleavage of covalently closed circular (ccc) ssDNA.
- Measuring DNA helicase activity.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Storage Class
12 - Non Combustible Liquids
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