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About This Item
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
peroxidase conjugate
Species reactivity:
-
Application:
—
Citations:
8
conjugate
peroxidase conjugate
antibody product type
primary antibodies
packaging
pack of 75 μL
manufacturer/tradename
Cytiva RPN1236
color
Colorless
shipped in
dry ice
storage temp.
−20°C
General description
Anti-GST HRP Conjugate.
Application
GST is a commonly used expression marker in cloning that is also used for purification and detection of fusion Proteins. This highly specific antibody to GST is conjugated to horseradish peroxidase and optimized for use in Western blotting with ECL™ Detection Reagents.
GST is a commonly used expression marker in cloning that is also used for purification and detection of fusion Proteins. This highly specific antibody to GST is conjugated to horseradish peroxidase and optimized for use in Western blotting with ECL™ Detection Reagents.
Features and Benefits
- Offers the advantages of speed, sensitivity, increased safety, and convenience when used with ECL™ detection systems. Application tested with ECL™ and ECL™ Plus reagents in western blotting for sensitive hard copy results
- Detection of GST is simplified because no secondary antibody is required
- The conjugate recognizes multiple epitopes of GST and therefore is not reliant on functional GST for fusion Protein detection. It has also been cross-absorbed to E. coli Proteins so that GST-fusion Proteins can be easily and specifically identified against an E. coli background.
- Binds with high affinity to GST fusion Proteins.
- Also suitable for colorimetric detection with diaminobenzidine (DAB) and tetramethylbenzidine (TMB).
- ECL™ optimized protocols included.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
GST Gene Fusion Vectors
A license for commercial use of GST Gene Fusion Vectors under US patent 5,654,176 and equivalent patents and patent applications in other countries must be obtained from Millipore Corp (formerly Chemicon International Inc).
A license for commercial use of GST Gene Fusion Vectors under US patent 5,654,176 and equivalent patents and patent applications in other countries must be obtained from Millipore Corp (formerly Chemicon International Inc).
ECL is a trademark of Cytiva
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Storage Class
12 - Non Combustible Liquids
Regulatory Information
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Articles
This page describes troubleshooting strategies in the detection of GST-tagged proteins.
Hannes Vanhaeren et al.
eLife, 9 (2020-03-27)
Protein ubiquitination is a very diverse post-translational modification leading to protein degradation or delocalization, or altering protein activity. In Arabidopsis thaliana, two E3 ligases, BIG BROTHER (BB) and DA2, activate the latent peptidases DA1, DAR1 and DAR2 by mono-ubiquitination at
Asparagine endopeptidase cleaves α-synuclein and mediates pathologic activities in Parkinson's disease.
Zhang Z, et al.
Nature Structural and Molecular Biology, 24(8), 632-632 (2017)
Ibtissam Talhaoui et al.
Nature communications, 9(1), 5016-5016 (2018-11-28)
The repair of double-stranded DNA breaks (DSBs) by homologous recombination involves the formation of branched intermediates that can lead to crossovers following nucleolytic resolution. The nucleases Mus81-Mms4 and Yen1 are tightly controlled during the cell cycle to limit the extent
Global Trade Item Number
| SKU | GTIN |
|---|---|
| GERPN1236 | 04061834084747 |