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GERPN3005

ECL Direct Nucleic Acid

Cytiva RPN3005, pack of 1 ea

Synonym(s):

DNA/RNA detection, HRP, chemiluminescent detection, luminol, module, reagent

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About This Item

NACRES:
NA.31
UNSPSC Code:
41105300
Storage temp.:
2-8°C
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packaging

pack of 1 ea

manufacturer/tradename

Cytiva RPN3005

storage temp.

2-8°C

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General description

ECL Direct Labeling Module.

Application

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Each system includes the following reagents, sufficient for labeling 5 to 10 μg nucleic acid and detecting 2000 to 4000 cm2 of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL Detection Reagents, and ECL Gold Hybridization Buffer.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

ECL is a trademark of Cytiva

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Storage Class

12 - Non Combustible Liquids

Regulatory Information

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Senia Rosales et al.
BMC microbiology, 10, 208-208 (2010-08-04)
Tuberculosis persists as a public health problem in Honduras. A better knowledge of the molecular characteristics of Mycobacterium tuberculosis strains will contribute to understand the transmission dynamics of the disease within the country. The aim of this study was to
H M Peng et al.
Molecular pharmacology, 54(4), 740-747 (1998-10-10)
The regulation of cytochrome P450 (CYP) 2E1, the ethanol-inducible isoform, is particularly complex. The level is affected by a variety of other foreign compounds, by insulin (as studied in several laboratories), and by triiodothyronine (T3), which has not been previously
C Couturier et al.
Arteriosclerosis, thrombosis, and vascular biology, 20(12), 2559-2565 (2000-12-16)
Type II secreted phospholipase A(2) (sPLA(2)) releases precursors of important inflammatory lipid mediators from phospholipids. Some observations have indicated that the sPLA(2), which has been implicated in chronic inflammatory conditions such as arthritis, contributes to atherosclerosis in the arterial wall.
Fátima C Pereira et al.
PLoS genetics, 9(10), e1003782-e1003782 (2013-10-08)
Endosporulation is an ancient bacterial developmental program that culminates with the differentiation of a highly resistant endospore. In the model organism Bacillus subtilis, gene expression in the forespore and in the mother cell, the two cells that participate in endospore
Akiko Uemori et al.
Anticancer research, 28(1A), 91-95 (2008-04-04)
The non-insecticidal Bacillus thuringiensis strain B0195 produces parasporin (PS) whose cytocidal activity is preferential for human cancer cells. This study identified two ps genes, ps1Aa3 and ps1Ab1, from the strain B0195. The former gene was 2,169-bp long, encoding an 81

Articles

Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.

描述被分子生物学家用于检测转印膜上蛋白质和核酸的方法的背景和实验方案。

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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