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GERPN3005

ECL Direct Nucleic Acid

Cytiva RPN3005, pack of 1 ea

Synonym(s):

DNA/RNA detection, HRP, chemiluminescent detection, luminol, module, reagent

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About This Item

UNSPSC Code:
41105300
NACRES:
NA.31
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packaging

pack of 1 ea

manufacturer/tradename

Cytiva RPN3005

storage temp.

2-8°C

Related Categories

General description

ECL Direct Labeling Module.

Application

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Each system includes the following reagents, sufficient for labeling 5 to 10 μg nucleic acid and detecting 2000 to 4000 cm2 of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL Detection Reagents, and ECL Gold Hybridization Buffer.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

ECL is a trademark of Cytiva

Pictograms

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Signal Word

Danger

Storage Class Code

12 - Non Combustible Liquids

Regulatory Information

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Senia Rosales et al.
BMC microbiology, 10, 208-208 (2010-08-04)
Tuberculosis persists as a public health problem in Honduras. A better knowledge of the molecular characteristics of Mycobacterium tuberculosis strains will contribute to understand the transmission dynamics of the disease within the country. The aim of this study was to
S Olschwang et al.
Journal of medical genetics, 38(6), 356-360 (2001-06-05)
Germline mutations of the STK11/LKB1 tumour suppressor gene (19p13.3) are responsible for Peutz-Jeghers syndrome (PJS), a rare genetic disorder, which is dominantly inherited. In addition to the typical hamartomatous gastrointestinal polyps and perioral pigmented lesions, PJS is also associated with
Shin-Ichi Miyata et al.
Microbiology (Reading, England), 149(Pt 8), 2243-2250 (2003-08-09)
Thymidylate kinase (TMK) catalyses the phosphorylation of dTMP to form dTDP in both the de novo and salvage pathways of dTTP synthesis in both prokaryotes and eukaryotes. Two homologues of bacterial thymidylate kinase genes were identified in a genomic library
Yasuko Kimura et al.
Yeast (Chichester, England), 19(16), 1437-1445 (2002-12-13)
We recently discovered, on the chromosome of Saccharomyces cerevisiae sigma 1278b, novel MPR1 and MPR2 genes required for resistance to a toxic analogue of L-proline, L-azetidine-2-carboxylic acid. The MPR genes, which were absent in the S. cerevisiae genome project strain
M Yamao et al.
Genes & development, 13(5), 511-516 (1999-03-11)
The Bombyx mori fibroin light (L)-chain gene was cloned and the green fluorescent protein (GFP) gene inserted into exon 7. The chimeric L-chain-GFP gene was used to replace the polyhedrin gene of Autographa californica nucleopolyhedrovirus (AcNPV). This recombinant virus was

Articles

Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.

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