GL0010
Golgi Isolation Kit
sufficient for 50 g (tissue)
Synonym(s):
Golgi Kit, Isolation Kit for Golgi
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
usage
sufficient for 50 g (tissue)
Quality Level
technique(s)
fractionation: suitable
shipped in
wet ice
storage temp.
2-8°C
General description
The Golgi Isolation Kit provides a method for isolating Golgi membranes from mammalian soft tissues by discontinuous density gradient. The degree of Golgi enrichment can be determined by assaying the acitivty of UDP-galactosyl transferase or by immunodetection of Golgi specific marker proteins like B-COP or GM130 using appropriate antibodies (Cat. No. G6160 and G7295, respectively). Separation from other organelles can be measured using the appropriate marker detection kits (Cat. No. CS0780, CYTOCOX1, CY0100 and CAT100).
Application
Golgi Isolation Kit may be used for the isolation of Golgi membranes from mammalian soft tissues by discontinuous density gradient.
Analysis Note
The Golgi Isolation kit was optimized using rat liver and tested on rat kidney, spleen, and heart.
Kit Components Also Available Separately
Product No.
Description
SDS
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 5 mL
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Regulatory Information
常规特殊物品
低风险生物材料
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When higher eukaryotic cells enter mitosis, membrane organization changes dramatically and traffic between membrane compartments is inhibited. Since membrane transport along microtubules is involved in secretion, endocytosis, and the positioning of organelles during interphase, we have explored whether the mitotic
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We investigated the direct effect of inositol 1,4,5-trisphosphate (IP(3)) and ryanodine receptor agonists on Ca(2+) release from vesicles of a rat liver Golgi apparatus (GA) enriched fraction, which were actively loaded with (45)Ca(2+). Results in GA were compared with those
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Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
亚细胞组分离心分离是一种常用技术，广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同，因此通过离心，可从温和均质的样品中轻松分离和纯化细胞器组分。
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