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Merck
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GLN1

Glutamine and Glutamate Determination Kit

Synonym(s):

L-glutamine enzymatic quantification kit

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About This Item

NACRES:
NA.75
UNSPSC Code:
12352207

Key Documents

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usage

 kit sufficient for 50 tests

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

UV/Vis spectroscopy: suitable

λmax

340 nm

application(s)

cell analysis
detection

storage temp.

2-8°C

Quality Level

200

Related Categories

Application

For enzymatic determination of L-glutamine and L-glutamate via reduction of NAD to NADH, measured spectrophotometrically at 339 nm.
The detection ranges for this kit are: 0.056 - 0.56 mM L-glutamate; 0.14 - 1.4 mM L-glutamine.
Glutamine and Glutamate Determination Kit has been used to determine the concentration of L-glutamine:
  • in minimum essential medium (MEM) medium
  • in the supernatant
  • in plasma

Biochem/physiol Actions

The Glutamine/Glutamate Determination Kit is designed for the spectrophotometric measurement of L-glutamine and/or L-glutamate via enzymatic deamination of L-glutamine and dehydrogenation of L-glutamate with conversion of NAD+ to NADH. This kit may be used for the spectrophotometric measurement of L-glutamine and/or L-glutamate in liquid preparations.

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Danger

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品
危险化学品
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Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCM5094
SLCM5095
SLCP4312
SLCJ9374
SLCK3051

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Tianyu Han et al.
Cell research, 28(6), 655-669 (2018-03-09)
Glutamine metabolism plays an important role in cancer development and progression. Glutaminase C (GAC), the first enzyme in glutaminolysis, has emerged as an important target for cancer therapy and many studies have focused on the mechanism of enhanced GAC expression
Expansion of human mesenchymal stromal cells on microcarriers: growth and metabolism
Schop D, et al.
Journal of Tissue Engineering and Regenerative Medicine, 4(2), 131-140 (2010)
Yingchao Lin et al.
BMC plant biology, 17(1), 247-247 (2017-12-21)
The trehalose (Tre) pathway has strong effects on growth and development in plants through regulation of carbon metabolism. Altering either Tre or trehalose 6-phosphate (T6P) can improve growth and productivity of plants as observed under different water availability. As yet
Patricia Martins Bock et al.
Molecular and cellular biochemistry, 411(1-2), 351-362 (2015-11-05)
In this work, we aimed to investigate the effects of long-term supplementations with L-glutamine or L-alanyl-L-glutamine in the high-fat diet (HFD)-fed B6.129SF2/J mouse model over insulin sensitivity response and signaling, oxidative stress markers, metabolism and HSP70 expression. Mice were fed
X Chang et al.
Clinical and experimental dermatology, 42(6), 614-621 (2017-06-10)
Malignant melanoma (MM) is an aggressive malignancy, which accounts for 80% of skin cancer-related deaths and is notably resistant to conventional chemotherapeutic agents. One of the most common treatments for melanoma is surgery, followed by various combinations of chemotherapy drugs.
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Glutamine Metabolism in Cancer Cells

Sigma article discusses tumor cell metabolic pathways, focusing on aerobic glycolysis and mitochondrial activity.

许多癌细胞中的谷氨酰胺代谢失调

本文介绍了增殖活性细胞为何需要碳源和氮源合成大分子。尽管大部分肿瘤细胞利用有氧糖酵解途径并分流线粒体氧化磷酸化代谢物，但许多肿瘤细胞表现出线粒体活性增加。

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