GLN1

Glutamine and Glutamate Determination Kit

Name: Glutamine and Glutamate Determination Kit
Brand: SIGMA

Synonym(s):

L-glutamine enzymatic quantification kit

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

Key Documents

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usage

kit sufficient for 50 tests

Quality Level

200

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

UV/Vis spectroscopy: suitable

λ_max

340 nm

application(s)

cell analysis
detection

storage temp.

2-8°C

Related Categories

Cell Analysis

Cell Culture & Analysis

Cell Migration & Invasion Assays

Molecular Biology & Functional Genomics

Application

For enzymatic determination of L-glutamine and L-glutamate via reduction of NAD to NADH, measured spectrophotometrically at 339 nm.
The detection ranges for this kit are: 0.056 - 0.56 mM L-glutamate; 0.14 - 1.4 mM L-glutamine.

Glutamine and Glutamate Determination Kit has been used to determine the concentration of L-glutamine:

in minimum essential medium (MEM) medium
in the supernatant
in plasma

Biochem/physiol Actions

The Glutamine/Glutamate Determination Kit is designed for the spectrophotometric measurement of L-glutamine and/or L-glutamate via enzymatic deamination of L-glutamine and dehydrogenation of L-glutamate with conversion of NAD⁺ to NADH. This kit may be used for the spectrophotometric measurement of L-glutamine and/or L-glutamate in liquid preparations.

Pictograms

GHS06,GHS08,GHS05,GHS09

Signal Word

Danger

Hazard Statements

H301 + H311,H314,H317,H330,H334,H350,H410

Precautionary Statements

P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

危险化学品

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Sensitization of melanoma cells to temozolomide by overexpression of microRNA 203 through direct targeting of glutaminase-mediated glutamine metabolism.

X Chang et al.

Clinical and experimental dermatology, 42(6), 614-621 (2017-06-10)

Malignant melanoma (MM) is an aggressive malignancy, which accounts for 80% of skin cancer-related deaths and is notably resistant to conventional chemotherapeutic agents. One of the most common treatments for melanoma is surgery, followed by various combinations of chemotherapy drugs.

Gankyrin drives metabolic reprogramming to promote tumorigenesis, metastasis and drug resistance through activating β-catenin/c-Myc signaling in human hepatocellular carcinoma.

Ruiqi Liu et al.

Cancer letters, 443, 34-46 (2018-12-07)

Gankyrin plays important roles in tumorigenicity and metastasis of hepatocellular carcinoma (HCC). We have for the first time investigated the effects of Gankyrin on glycolysis and glutaminolysis both in vitro and in vivo, including in patient-derived xenografts. We reported Gankyrin

L-Glutamine and L-glutamate: UV-method with glutaminase and glutamate dehydrogenase.

Lund, P. et al.

Methods of Enzymatic Analysis, 8, 357-363 (1986)

N-Myc downstream-regulated gene 2 restrains glycolysis and glutaminolysis in clear cell renal cell carcinoma.

Wei Shi et al.

Oncology letters, 14(6), 6881-6887 (2017-11-23)

Glycolysis and glutaminolysis are heavily involved in the metabolic reprogramming of cancer cells. The activation of oncogenes and inactivation of tumor suppressor genes has a marked effect on the cellular metabolic processes glycolysis and glutaminolysis. N-Myc downstream-regulated gene 2 (NDRG2)

Monitoring for overreaching in rugby league players

Coutts AJ, et al.

European Journal of Applied Physiology, 99(3), 313-324 (2007)

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