H0160
HIV-1 gag
≥95% (SDS-PAGE), recombinant, expressed in E. coli, Galactosidase-tagged
Synonym(s):
p24 HIV antigen
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About This Item
Assay:
≥95% (SDS-PAGE)
Recombinant:
expressed in E. coli
Mol wt:
39 kDa (plus 114 kDa β-Gal tag)
recombinant
expressed in E. coli
Quality Segment
assay
≥95% (SDS-PAGE)
mol wt
39 kDa (plus 114 kDa β-Gal tag)
concentration
1 mg/mL
UniProt accession no.
storage temp.
−20°C
Gene Information
human immunodeficiency virus 1 ... gag(155030)
General description
Contains amino acid residues [77-436] of HIV-1 gag antigen.
The human immunodeficiency virus 1 (HIV−1) gag antigen is a recombinant 39 kDa protein encoded by the GAG gene of the human immunodeficiency virus (HIV) type I expressed in and isolated from Escherichia coli. It has a β−galactosidase tag (114 kDa) on the N−terminus. HIV-1 Gag exists as monomers or lower-order oligomers in the cytosol. At the plasma membrane, it forms higher-order multimers.
Application
HIV-1 gag has been used in the coculture with an autologous cluster of differentiation T (CD4+ T) to study the ability of monocyte-derived dendritic cells (MDC1) to induce human immunodeficiency virus 1 (HIV−1) latency reversal (LR).
Biochem/physiol Actions
Positive control for HIV antibodies.
The human immunodeficiency virus 1 (HIV−1) gag (p24) antigen reacts strongly with HIV positive serum and may be used as a positive control for evaluating antibodies to HIV. At transcription sites, HIV-1 Gag creates ribonucleoprotein complexes with unspliced viral RNA. The HIV-1 Gag protein is accountable for packaging genomic RNA (gRNA) and the assembly of immature viral particles.
Physical form
Solution in 8 M urea, 20 mM Tris-HCl, pH 8.0, 10 mM 2-mercaptoethanol.
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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