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Merck
CN

H0527

HEPES potassium salt

≥99.5% (titration)

Synonym(s):

Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-monopotassium salt, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid potassium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) potassium salt

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About This Item

Empirical Formula (Hill Notation):
C8H17KN2O4S
CAS Number:
82207-62-3
Molecular Weight:
276.39
NACRES:
NA.25
PubChem Substance ID:
24895405
UNSPSC Code:
12161700
MDL number:
MFCD00043438

Key Documents

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InChI key

KWURIFKZTJGMFD-UHFFFAOYSA-M

SMILES string

[K+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.K/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

assay

≥99.5% (titration)

form

powder

useful pH range

6.8-8.2

pKa 

7.5

solubility

water: 0.5 g/mL, clear, colorless

Quality Level

200

Related Categories

General description

HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid) is a widely used Good’s buffer in biochemical and biological research applications. As a zwitterionic buffer, it stabilizes the pH of the solution even when small amounts of acid or base are added.

Application

HEPES potassium salt has been used as a buffering agent in nanopore fabrication and electrolyte preparations. It has also been used as a component of cell lysis buffer.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000511081
0000511082
0000511082
0000511081
0000495740

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Use of a solid-state nanopore for profiling the transferrin receptor protein and distinguishing between transferrin receptor and its ligand protein
O'Donohue M, et al.
Electrophoresis (2023)
Equilibrium studies, synthesis and characterisation of zwitterionic buffer (HEPES and HEPPS) dimethyltin (IV) complexes
Mohamed M
Main Group Chemistry, 63-84 (2007)
Complexation of copper by zwitterionic aminosulfonic (good) buffers
Mash HE, et al.
Analytical Chemistry (2003)
Determining the location of an Arabidopsis chloroplast protein using in vitro import followed by fractionation and alkaline extraction
Chu CC and Li HM
Methods in Molecular Biology (2011)
Jugal Saharia et al.
Electrophoresis, 43(5-6), 785-792 (2022-01-13)
Electrolyte chemistry plays an important role in the transport properties of analytes through nanopores. Here, we report the translocation properties of the protein human serum transferrin (hSTf) in asymmetric LiCl salt concentrations with either positive (Ctrans /Ccis < 1) or
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