H0527
HEPES 钾盐
≥99.5% (titration)
别名:
哌嗪乙磺酸，4-(2-羟乙基)-磷酸二氢钾, 4-(2-羟乙基)哌嗪-1-乙磺酸 钾盐, N-(2-羟乙基)哌嗪-N′-(2-乙磺酸) 钾盐
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关于此项目
经验公式（希尔记法）:
C8H17KN2O4S
化学文摘社编号:
分子量:
276.39
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12161700
MDL number:
InChI key
KWURIFKZTJGMFD-UHFFFAOYSA-M
SMILES string
[K+].OCCN1CCN(CC1)CCS([O-])(=O)=O
InChI
1S/C8H18N2O4S.K/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
assay
≥99.5% (titration)
form
powder
useful pH range
6.8-8.2
pKa
7.5
solubility
water: 0.5 g/mL, clear, colorless
Quality Level
General description
HEPES（4-(2-羟乙基）哌嗪-1-乙磺酸）是生物化学和生物研究应用中广泛使用的良好’缓冲剂。作为一种带电离子的缓冲剂，它可以在加入少量酸或碱时稳定溶液的pH值。
Application
HEPES钾盐已被用作纳米孔制备和电解质制备中的缓冲剂。它还可被用作细胞裂解液的组分。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Determining the location of an Arabidopsis chloroplast protein using in vitro import followed by fractionation and alkaline extraction
Chu CC and Li HM
Methods in Molecular Biology (2011)
Equilibrium studies, synthesis and characterisation of zwitterionic buffer (HEPES and HEPPS) dimethyltin (IV) complexes
Mohamed M
Main Group Chemistry, 63-84 (2007)
Use of a solid-state nanopore for profiling the transferrin receptor protein and distinguishing between transferrin receptor and its ligand protein
O'Donohue M, et al.
Electrophoresis (2023)
Complexation of copper by zwitterionic aminosulfonic (good) buffers
Mash HE, et al.
Analytical Chemistry (2003)
Shankar Dutt et al.
Analytical chemistry, 95(13), 5754-5763 (2023-03-18)
Thin membranes are highly sought-after for nanopore-based single-molecule sensing, and fabrication of such membranes becomes challenging in the ≲10 nm thickness regime where a plethora of useful molecule information can be acquired by nanopore sensing. In this work, we present
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