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H1029

Monoclonal Anti-polyHistidine antibody produced in mouse

Name: Monoclonal Anti-polyHistidine antibody produced in mouse
Brand: SIGMA

clone HIS-1, ascites fluid

Synonym(s):

Monoclonal Anti-polyHistidine, Monoclonal 6 His epitope tag, Monoclonal 6xHis-tag, Monoclonal HHHHHH epitope tag, Monoclonal Hexa His tag, Monoclonal His-tag, Monoclonal His6 tag, Monoclonal Histidine tagged, Monoclonal Poly-His-tag

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About This Item

NACRES:

NA.56

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

HIS-1, monoclonal

Application:

DB, ELISA (i), IP, WB

Citations:

448

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biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

HIS-1, monoclonal

form

liquid

technique(s)

dot blot: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 1:3,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein

isotype

IgG2a

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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Related Categories

Antibodies - Primary, Secondary & Recombinant Monoclonal

Antibodies by Application

Primary Antibodies

General description

The Monoclonal Anti-polyHistidine (mouse IgG2a) antibody recognizes native or denatured, reduced forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The antibody preferentially recognizes the N-terminal tagged fusion protein and is reactive with fusion proteins expressed by the prokaryotic expression vectors pET, pRSET, and pTrc.

Immunogen

recombinant polyHistidine tagged fusion protein.

Application

Antibody suitable for immunoblotting, dot blot assays, ELISA and immunocytochemistry. Monoclonal Anti-polyHistidine antibody produced in mouse has been used in Western blotting.

Biochem/physiol Actions

The IgG2a (Immunoglobulin G 2a) gene actively takes part in stimulating autoimmunity in mice. The gene is found to be highly expressed in virus infection.

The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.

Physical form

Suppled as ascites fluid with 15mM sodium azide.

Other Notes

Recommended dilution for immunoblotting is 1:3000

Legal Information

This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.

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Storage Class

10 - Combustible liquids

Regulatory Information

常规特殊物品

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Certificates of Analysis (COA)

Lot/Batch Number

0000462557

0000439628

0000352386

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A novel Ca2+-binding protein that can rapidly transduce auxin responses during root growth.

Ora Hazak et al.

PLoS biology, 17(7), e3000085-e3000085 (2019-07-12)

Signaling cross talks between auxin, a regulator of plant development, and Ca2+, a universal second messenger, have been proposed to modulate developmental plasticity in plants. However, the underlying molecular mechanisms are largely unknown. Here, we report that in Arabidopsis roots

Contribution of IRF5 in B cells to the development of murine SLE-like disease through its transcriptional control of the IgG2a locus.

Savitsky D A, et al.

Proceedings of the National Academy of Sciences of the USA (2010)

Association of a novel pre-ribosomal complex in Trypanosoma brucei.

Wang L

Eukaryotic Cell (2013)

Proteolysis of HCF-1 by Ser/Thr glycosylation-incompetent O-GlcNAc transferase:UDP-GlcNAc complexes.

Vaibhav Kapuria et al.

Genes & development, 30(8), 960-972 (2016-04-09)

In complex with the cosubstrate UDP-N-acetylglucosamine (UDP-GlcNAc),O-linked-GlcNAc transferase (OGT) catalyzes Ser/ThrO-GlcNAcylation of many cellular proteins and proteolysis of the transcriptional coregulator HCF-1. Such a dual glycosyltransferase-protease activity, which occurs in the same active site, is unprecedented and integrates both reversible

A variant upstream of IFNL3 (IL28B) creating a new interferon gene IFNL4 is associated with impaired clearance of hepatitis C virus.

Prokunina-Olsson L, et al.

Nature Genetics, 45(2), 164-164 (2013)

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Global Trade Item Number

SKU	GTIN
H1029-.2ML	04061838446121
H1029-100UL	04061838446138
H1029-.5ML	04061833663790

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