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H1136

Hyaluronidase from Streptomyces hyalurolyticus

Name: Hyaluronidase from <I>Streptomyces hyalurolyticus</I>
Brand: SIGMA

Synonym(s):

Hyaluronate Lyase from Streptomyces hyalurolyticus

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About This Item

CAS Number:

37259-53-3

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

253-430-8

MDL number:

MFCD00131347

EC Number:

4.2.2.1 (BRENDA, IUBMB)

Biological source:

Streptomyces sp. (S. hyalurolyticus)

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biological source

Streptomyces sp. (S. hyalurolyticus)

Quality Level

200

conjugate

conjugate (Glucosaminoglycan)

form

film (Clear)

packaging

ampule of 300 × units

storage temp.

−20°C

Related Categories

Glycobiology Profiling Tools

Protein Biology

Proteins & Enzymes

General description

Hyaluronidase is widely present in nature. In humans, it is present in a number of organs and body fluids. Hyaluronidase is found in the secretions of virus, bacteriophage, fungi and bacteria. It is also present in nematode and leeches secretions. Snakes and scorpions produce hyaluronidase in their venom. Hyaluronidase is also secreted by bees, spiders, wasps, hornets, caterpillars, fishes and lizards.

Application

Hyaluronidase from Streptomyces hyalurolyticus has been used:

in the preparation of DMEM/F-12 (Dulbecco′s modified eagle medium/nutrient mixture F-12) media for the isolation and purification of single cells from dissociated tumour tissue
as a component of digestion solution for the derivation of germline stem cells from testicular tissue

Biochem/physiol Actions

Hyaluronidase is an endoglycosidase and performs its function along with exoglycosidases, like β-glucuronidase and β-N-acetyl glucosaminidase. Hyaluronidase regulates the hyaluronic acid turnover.

Hyaluronate lyase cleaves hyaluronic acid at the β-D-GlcNAc-(1→4)-β-D-GlcA bond, yielding 3-(4-deoxy-β-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine tetra- and hexasaccharides. Unlike other hyaluronidases, this enzyme is specific for hyaluronic acid and is inactive with chondroitin and chondroitin sulfate.

Other Notes

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

常规特殊物品

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Certificates of Analysis (COA)

Lot/Batch Number

0000536365

0000459016

0000462490

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Binding of trastuzumab to ErbB2 is inhibited by a high pericellular density of hyaluronan.

Tímea Váradi et al.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 60(8), 567-575 (2012-05-09)

Although trastuzumab is an efficient drug, primary and acquired resistance is a challenging problem. The authors have previously shown in mouse xenograft experiments that masking ErbB2 by hyaluronan leads to diminished binding of the antibody and consequent resistance. In the

Crystal structure of hyaluronidase, a major allergen of bee venom

Markovic H, et al.

Structure, 8(10), 1025-1035 (2000)

Force Transmission Between the Gastrocnemius and Soleus Sub-Tendons of the Achilles Tendon in Rat.

Connor C Gains et al.

Frontiers in bioengineering and biotechnology, 8, 700-700 (2020-08-09)

The Achilles tendon (AT) is comprised of three distinct sub-tendons bound together by the inter-subtendon matrix (ISTM). The interactions between sub-tendons will have important implications for AT function. The aim of this study was to investigate the extent to which

Decreased pericellular matrix production and selection for enhanced cell membrane repair may impair osteocyte responses to mechanical loading in the aging skeleton.

Mackenzie L Hagan et al.

Aging cell, 19(1), e13056-e13056 (2019-11-20)

Transient plasma membrane disruptions (PMD) occur in osteocytes with in vitro and in vivo loading, initiating mechanotransduction. The goal here was to determine whether osteocyte PMD formation or repair is affected by aging. Osteocytes from old (24 months) mice developed fewer

CD44v8-10 is a cancer-specific marker for gastric cancer stem cells

Lau WM, et al.

Cancer Research, canres-ca2309 (2014)

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Protocols

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

透明质酸酶的酶法测定 (3.2.1.35)

在测定透明质酸酶活性时，使用了一种在600 nm处的比浊测定法进行测定。在pH 5.35、37 °C下，一单位的透明质酸酶活性可每分钟引起0.330的吸光值改变。

Global Trade Item Number

SKU	GTIN
H1136-1AMP	04061833663936
H1136-5X1AMP	04061833663950

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