H1404
Hepatocyte Growth Factor human
>95% (SDS-PAGE), recombinant, expressed in Baculovirus infected High-5 cells, lyophilized powder, suitable for cell culture
Synonym(s):
HGF, Hepatopoietin A, Scatter Factor
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About This Item
Biological source:
human
Recombinant:
expressed in Baculovirus infected High-5 cells
Assay:
>95% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
~80 kDa
Impurities:
<1.0 EU/μg
Product Name
Hepatocyte Growth Factor human, HGF, recombinant, expressed in Baculovirus infected High-5 cells, suitable for cell culture
biological source
human
recombinant
expressed in Baculovirus infected High-5 cells
assay
>95% (SDS-PAGE)
form
lyophilized powder
potency
1.0-20.0 ng/mL
quality
endotoxin tested
mol wt
~80 kDa
packaging
pkg of 5x5 μg, pkg of 5 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
<1.0 EU/μg
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... HGF(3082)
General description
Recombinant human Hepatocyte Growth Factor (HGF) is supplied as a lyophilized protein suitable for mammalian cell culture research. HGF is a multifunctional growth factor that signals through the c‑Met receptor and is widely used in in vitro cell studies.
Application
HGF has been used in mammalian cell culture research applications, including epithelial, endothelial, and hepatocyte‑related in vitro model systems.
Biochem/physiol Actions
Hepatocyte Growth Factor (HGF) exhibits mitogenic, motogenic, and morphogenic activity in vitro across multiple cell types through activation of c‑Met receptor‑mediated signaling pathways.
Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of cell types including endothelial and epithelial cells, melanocytes, and keratinocytes. It also mediates epithelial morphogenesis. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.
Physical form
Lyophilized from a 0.2 μm filtered solution in 20 mM NaH2PO4 and 500 mM NaCl, pH 7.0 with 50 μg BSA per 1 μg HGF
Preparation Note
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. After reconstitution, this solution can be stored at 2-8 °C for up to one month or at -20 °C to -70 °C for up to three months. Us a manual defrost freezer and avoid repeated freeze-thaw cycles.
Analysis Note
The biological activity is measured by its ability to induce IL-11 secretion by Saos-2 human osieosarcoma cells.
Other Notes
The NSO-expressed recombinant HGF is greater than 95% heterodimeric HGF. The insect cell line-expressed recombinant HGF is a mixture of single chain HGF (predominant) with some heterodimeric HGF. Single chain HGF and heterodimeric HGF are equally active in in vitro assays.
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Storage Class
11 - Combustible Solids
WGK
WGK 2
PPE (personal protective equipment)
dust mask type N95 (US), Eyeshields, Gloves
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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