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H1506

3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni

≥15 units/mg protein

Synonym(s):

3α-Hydroxysteroid:NAD(P)+ oxidoreductase

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About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-839-5
MDL number:
EC Number:
Specific activity:
≥15 units/mg protein
Biological source:
bacterial (Pseudomonas testosteroni)
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biological source

bacterial (Pseudomonas testosteroni)

Quality Level

form

lyophilized solid

specific activity

≥15 units/mg protein

purified by

chromatography

composition

Protein, ≥25%

foreign activity

β-hydroxysteroid dehydrogenase ≤0.5%, alcohol dehydrogenase ≤0.05%

storage temp.

−20°C

Application

3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosterone has been used:
  • in the preparation of composite electrode platform to for the determination of androsterone
  • in potassium phosphate buffer for the bioluminescent assay of bile acids in serum
  • for total bile salts assay using an enzymatic method

Biochem/physiol Actions

3α-hydroxysteroid dehydrogenase (3α-HSD) may help to modulate the occupancy of the androgen receptor (AR) in androgen target tissues. It may also act in hindering androgen action.

Physical form

Lyophilized powder containing potassium phosphate buffer salt, EDTA, and DL-dithiothreitol

Other Notes

One unit will oxidize 1.0 μmole of androsterone per min at pH 8.9 at 25 °C in the presence of β-NAD+.


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Xiang Li et al.
Archives of pharmacal research, 30(7), 812-815 (2007-08-21)
Two novel lactones, Cephalosporolides H (1) and 1 (2), were isolated from the lyophilized culture broth of the marine-derived fungus, Penicillium sp. The structures were elucidated on the basis of extensive 1D- and 2D-NMR, as well as HRESI-MS spectroscopic analyses.
Bin Li et al.
Proteins, 71(2), 670-683 (2007-11-03)
Experimentally determined protein tertiary structures are rapidly accumulating in a database, partly due to the structural genomics projects. Included are proteins of unknown function, whose function has not been investigated by experiments and was not able to be predicted by
Bioluminescent assay for total bile acids in serum with use of bacterial luciferase.
Styrelius I, et al.
Clinical Chemistry, 29(6), 1123-1127 (1983)



Global Trade Item Number

SKUGTIN
H1506-50UN04061833666623