H1506
3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni
≥15 units/mg protein
Synonym(s):
3α-Hydroxysteroid:NAD(P)+ oxidoreductase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-839-5
MDL number:
Specific activity:
≥15 units/mg protein
Biological source:
bacterial (Pseudomonas testosteroni)
biological source
bacterial (Pseudomonas testosteroni)
Quality Segment
form
lyophilized solid
specific activity
≥15 units/mg protein
purified by
chromatography
composition
Protein, ≥25%
foreign activity
β-hydroxysteroid dehydrogenase ≤0.5%, alcohol dehydrogenase ≤0.05%
storage temp.
−20°C
Application
3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosterone has been used:
- in the preparation of composite electrode platform to for the determination of androsterone
- in potassium phosphate buffer for the bioluminescent assay of bile acids in serum
- for total bile salts assay using an enzymatic method
Biochem/physiol Actions
3α-hydroxysteroid dehydrogenase (3α-HSD) may help to modulate the occupancy of the androgen receptor (AR) in androgen target tissues. It may also act in hindering androgen action.
Physical form
Lyophilized powder containing potassium phosphate buffer salt, EDTA, and DL-dithiothreitol
Other Notes
One unit will oxidize 1.0 μmole of androsterone per min at pH 8.9 at 25 °C in the presence of β-NAD+.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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