H4042
HGH active human
Animal-component free, recombinant, expressed in Nicotiana, >97% (SDS-PAGE)
Synonym(s):
GH, GH-N, Growth hormone, Growth hormone 1, Pituitary growth hormone
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About This Item
UNSPSC Code:
51111800
biological source
human
recombinant
expressed in Nicotiana
assay
>97% (SDS-PAGE)
form
lyophilized
potency
≤0.1 ng/mL
mol wt
22.9 kDa (205 amino residues)
packaging
pkg of 50 μg
storage condition
avoid repeated freeze/thaw cycles
impurities
Endotoxin, tested (LAL test, < 0.04 EU/ ug protein)
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... GH1(2688)
General description
GH is a member of the somatotropin/prolactin family of hormones which play an important role in growth control.
GH is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature.
Biochem/physiol Actions
GH (growth hormone) is mainly crucial at puberty for the mammary gland development. In epithelioid breast cancer, autocrine GH enhances epithelial-mesenchymal transition. In addition, GH is needed for lipid metabolism and adipose tissue remodelling.
Physical form
Lyophilized from a PBS 0.05 M buffer at pH 7.5
Preparation Note
Lyophilized protein should be reconstituted in water to a concentration of 50 ng/μl. Due to the protein nature, dimmers and multimers may be observed.
Analysis Note
The biological activity of human Growth Hormone is measured by cell proliferation using Nb2-11 cells.
ED50 ≤ 0.04-0.1 ng/mL
ED50 ≤ 0.04-0.1 ng/mL
Other Notes
Extinction Coefficient: E0.1% = 0.77 (A280 nm)
p.I: 5.98
p.I: 5.98
Storage Class
13 - Non Combustible Solids
wgk
WGK 2
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