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H4502

Hexokinase from Saccharomyces cerevisiae

Name: Hexokinase from <I>Saccharomyces cerevisiae</I>
Brand: SIGMA

Type F-300, lyophilized powder, ≥130 units/mg protein (biuret)

Synonym(s):

Hexokinase D, Hexokinasetype IV, ATP:D-Hexose-6-phosphotransferase, Hexokinase from yeast

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About This Item

CAS Number:

9001-51-8

UNSPSC Code:

12352204

eCl@ss:

32160410

EC Number:

232-611-5

NACRES:

NA.54

MDL number:

MFCD00131321

EC Number:

2.7.1.1 (BRENDA, IUBMB)

Specific activity:

≥130 units/mg protein (biuret)

Biological source:

Saccharomyces cerevisiae

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Properties

biological source

Saccharomyces cerevisiae

Quality Segment

200

type

Type F-300

form

lyophilized powder

specific activity

≥130 units/mg protein (biuret)

mol wt

110 kDa

purified by

crystallization

storage condition

(Keep container tightly closed in a dry and well-ventilated place)

color

white

anion traces

sulfate (SO₄^2-): free

UniProt accession no.

P04806, P04807

foreign activity

ATPase, myokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconic dehydrogenase and phosphoglucose isomerase ≤0.01%

storage temp.

−20°C

Gene Information

bakers yeast ... HXK1(850614), HXK2(852639)

Description

General description

Research area: Cell Signaling

The hexokinase 1 (HXK1) gene is mapped to the Saccharomyces cerevisiae chromosome VI. HXK1 is ubiquitously expressed in mammalian tissues and is abundant in the brain, erythrocytes, lymphocytes, and fibroblasts.

Application

Hexokinase has been used:

for digesting glucose for measuring glucose content in the root and stem samples of Quercus velutina
to treat ADP (adenosine diphosphate) solution along with D-glucose to remove the contaminating ATP (adenosine triphosphate)
in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer (pH 7.5) for ADP-[13CU]glucose synthesis

Biochem/physiol Actions

Hexokinase catalyzes the rate-limiting first step of glycolysis, which involves the phosphorylation of glucose.

Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source.

The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C).
D-fructose K_M: 0.33 mM
D-glucose K_M: 0.12 mM
D-mannose K_M: 0.05 mM

Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.

Molecular Weight:~54 kDa (monomer)
~110 kDa (dimer)
Optimal pH: 7.5 to 9.0
Extinction Coefficient: E^1% = 8.85 (PI) and 9.47 (PII) at 280 nm

Activators: Hexokinase requires Mg²⁺ ions (K_M = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds.

Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg²⁺ and 4-chloromercuribenzoate)

Physical form

Lyophilized powder containing approx. 15% sodium citrate

Preparation Note

Reconstitute with water or citrate buffer, pH 7.0. Studies have shown excellent stability during repeated freezing and thawing over a period of at least 30 days.

Analysis Note

A mixture of isoenzymes.

Other Notes

One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c