H5416
FLT3 Ligand human
HumanKine®, recombinant, expressed in HEK 293 cells, suitable for cell culture
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About This Item
Biological source:
human
Recombinant:
expressed in HEK 293 cells
Assay:
≥95% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
monomer 24-30 kDa (glycosylated)
Impurities:
≤1 EU/mg
Product Name
FLT3 Ligand human, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture
biological source
human
Quality Level
recombinant
expressed in HEK 293 cells
assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
≤0.8 ng/mL EC50
quality
endotoxin tested
mol wt
monomer 24-30 kDa (glycosylated)
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/mg
storage temp.
−20°C
General description
Human FMS-like tyrosine kinase 3 (FLT3) ligand is a homodimer consisting of two short-chain α-helical bundles. The molecular weight of FLT3 ligand is 44 kDa.
Biochem/physiol Actions
FLT3 ligand binds to the cell-bound tyrosine kinase receptor and activates them.
HumanKine FLT3 Ligand is expressed in human HEK 293 cells as a glycosylated monomer with an apparent molecular mass of 24-30 kDa. HumanKine FLT3 Ligand in human HEK 293 cells contributes to glycosylation.FLT3 ligand is a hematopoietic cytokine that regulates the proliferation of early hematopoietic cells. It has been shown to synergize with a variety of cytokines to stimulate the growth and differentiation of early hematopoietic cells. The FLT3 ligand also stimulates the expansion of monocytes and immature dendritic cells, and induces early B cell lineage differentiation and NK cell growth.
Physical form
Lyophilized from a 0.2 μm filtered solution of 1x PBS.
Analysis Note
The specific activity was determined by the dose-dependent stimulation of the proliferation of the human acute myeloid leukemia cell line OCI-AML5.
Legal Information
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Related Content
Instructions
Flt3 ligand structure and unexpected commonalities of helical bundles and cystine knots
Savvides S N, et al.
Nature Structural and Molecular Biology, 7(6), 486-486 (2000)
Structure-function analysis of FLT3 ligand-FLT3 receptor interactions using a rapid functional screen
Graddis T, et al.
The Journal of Biological Chemistry, 273(28), 17626-17633 (1998)
Chunaram Choudhary et al.
International journal of hematology, 82(2), 93-99 (2005-09-09)
Activating mutations of Fms-like tyrosine kinase 3 (Flt3) are the most common genetic lesions in acute myeloid leukemia (AML) and are present in approximately one third of AML patients. The 2 classes of Flt3 mutations are internal tandem duplications in
Global Trade Item Number
| SKU | GTIN |
|---|---|
| H5416-10UG | 04061833799406 |