H5791
Hepatocyte Growth Factor human
HumanKine®, ≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
Synonym(s):
HGF, Hepatopoietin A, Scatter Factor
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About This Item
Biological source:
human
Recombinant:
expressed in HEK 293 cells
Assay:
≥95% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
monomer 70 kDa (glycosylated)
Impurities:
≤1 EU/mg
Product Name
Hepatocyte Growth Factor human, HGF, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture
biological source
human
Quality Segment
recombinant
expressed in HEK 293 cells
assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
≤20 ng/mL EC50
quality
endotoxin tested
mol wt
monomer 70 kDa (glycosylated)
packaging
pkg of 5x10 μg, pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/mg
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... HGF(3082)
General description
Recombinant human hepatocyte growth factor (HGF) is expressed in HEK 293 cells and supplied as a glycosylated lyophilized protein suitable for mammalian cell culture research. HGF is the ligand for the c‑Met receptor and is widely studied in in vitro cell signaling and growth models.
Application
Recombinant HGF has been used in mammalian cell culture applications, including epithelial, endothelial, and hepatocyte‑related in vitro research models.
Biochem/physiol Actions
Hepatocyte Growth Factor (HGF) is a multifunctional growth factor that exhibits mitogenic and motogenic activity in vitro and mediates cellular responses through activation of the c‑Met receptor signaling pathway.
Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of cell types including endothelial and epithelial cells, melanocytes, and keratinocytes. It also mediates epithelial morphogenesis. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.
Physical form
Lyophilized from a 0.2 μm filtered solution of 1x PBS.
Preparation Note
HumanKine HGF is expressed as a glycosylated 70 kDa single chain monomer in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.
Analysis Note
The specific activity was determined by the dose-dependent stimulation of the proliferation of the monkey epithelial cell line 4MBr-5.
Other Notes
The NSO-expressed recombinant HGF is greater than 95% heterodimeric HGF. The insect cell line-expressed recombinant HGF is a mixture of single chain HGF (predominant) with some heterodimeric HGF. Single chain HGF and heterodimeric HGF are equally active in in vitro assays.
Legal Information
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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