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Merck
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H7787

HIS-Select® Spin Columns

Synonym(s):

His-Tag Spin Columns

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About This Item

NACRES:
NA.56
UNSPSC Code:
41106500
Pore size:
100 nm pore size
Packaging:
pkg of 10 ea
pkg of 50 ea

Key Documents

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shelf life

2 yr at 2‑8 °C

packaging

pkg of 10 ea
pkg of 50 ea

pore size

100 nm pore size

capacity

>0.5 mg/column

storage temp.

2-8°C

Quality Level

200

Related Categories

Application

Pre-packed and ready-to-use HIS-Select Spin Columns allow for fast and consistent purification of small-scale crude cell extracts containing histidine-tagged proteins. The spin columns contain a matrix of silica particles and uses the HIS-Select patented non-charged, hydrophilic nickel chelating linkage chemistry. HIS-Select Spin Columns are highly selective for histidine-tagged proteins and exhibit very low non-specific binding of other proteins. These convenient Spin Columns allow for fast, one-step purification for the isolation of at least 150 μg of histidine-tagged protein in less than 15 minutes.

Features and Benefits

  • High Selectivity for higher purity
  • Convenient and Ready-to-Use
  • Consistent protein binding each use
  • Compatible with CelLytic™ cell lysis reagents

General description

Kit contains 3 collection tubes for every spin column.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000439359
0000439359
0000437261
0000437261
SLCQ6970

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the binding capacity of the HIS-Select® Spin Column, Product H7787?

    The binding capacity of a HIS-Select Spin Column is >500 ug per column as determined witha ~30 kDa histidine tagged protein recovered under high levels of protein expression.

  4. Can HIS-Select® Spin Column, Product H7787, be re-used?

    We do not recommend re-using the columns. Each column is intended for one time use.

  5. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  6. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

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    Ask a Scientist here.

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Fiorentino,M et al.
International Journal of Molecular Medicine, 23(3), 387-397 (2009)
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