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Merck
CN

H7787

Millipore

HIS-Select® Spin Columns

Synonym(s):

His-Tag Spin Columns

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

Key Documents

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shelf life

2 yr at 2‑8 °C

packaging

pkg of 10 ea
pkg of 50 ea

pore size

100 nm pore size

capacity

>0.5 mg/column

storage temp.

2-8°C

Related Categories

General description

Kit contains 3 collection tubes for every spin column.

Application

Pre-packed and ready-to-use HIS-Select Spin Columns allow for fast and consistent purification of small-scale crude cell extracts containing histidine-tagged proteins. The spin columns contain a matrix of silica particles and uses the HIS-Select patented non-charged, hydrophilic nickel chelating linkage chemistry. HIS-Select Spin Columns are highly selective for histidine-tagged proteins and exhibit very low non-specific binding of other proteins. These convenient Spin Columns allow for fast, one-step purification for the isolation of at least 150 μg of histidine-tagged protein in less than 15 minutes.

Features and Benefits

  • High Selectivity for higher purity
  • Convenient and Ready-to-Use
  • Consistent protein binding each use
  • Compatible with CelLytic™ cell lysis reagents

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000439359
0000439359
0000437261
0000437261
SLCQ6970

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the binding capacity of the HIS-Select® Spin Column, Product H7787?

    The binding capacity of a HIS-Select Spin Column is >500 ug per column as determined witha ~30 kDa histidine tagged protein recovered under high levels of protein expression.

  4. Can HIS-Select® Spin Column, Product H7787, be re-used?

    We do not recommend re-using the columns. Each column is intended for one time use.

  5. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  6. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

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    Ask a Scientist here.

Maureen J Bibb et al.
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The chaplin and rodlin proteins together constitute the major components of the hydrophobic sheath that coats the aerial hyphae and spores in Streptomyces, and mutants lacking the chaplins are unable to erect aerial hyphae and differentiate on minimal media. We
Wenbo Li et al.
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Choroidal neovascularization (CNV) in age-related macular degeneration usually causes blindness. We established a novel targeted inhibitor for CNV in age-related macular degeneration. The inhibitor CR2-sFlt 1 comprises a CR2-targeting fragment and an anti-vascular endothelial growth factor (VEGF) domain (sFlt 1).
Maria A Schumacher et al.
Nucleic acids research, 46(14), 7405-7417 (2018-06-16)
Streptomyces are filamentous bacteria with a complex developmental life cycle characterized by the formation of spore-forming aerial hyphae. Transcription of the chaplin and rodlin genes, which are essential for aerial hyphae production, is directed by the extracytoplasmic function (ECF) σ
Mutational analysis of the cleavage of the cancer-associated laminin receptor by stromelysin-3 reveals the contribution of flanking sequences to site recognition and cleavage efficiency
Fiorentino,M et al.
International Journal of Molecular Medicine, 23(3), 387-397 (2009)
Ozan Gundogdu et al.
Journal of bacteriology, 193(16), 4238-4249 (2011-06-07)
Campylobacter jejuni is the leading bacterial cause of human gastroenteritis worldwide. Despite stringent microaerobic growth requirements, C. jejuni is ubiquitous in the aerobic environment and so must possess regulatory systems to sense and adapt to external stimuli, such as oxidative
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