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Merck
CN

H8889

Histopaque®-1077 Hybri-Max™

liquid, sterile-filtered, BioReagent, suitable for hybridoma

Synonym(s):

Histopaque 1077

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About This Item

NACRES:
NA.75
UNSPSC Code:
12352201

Key Documents

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Product Name

Histopaque®-1077 Hybri-Max™, liquid, sterile-filtered, BioReagent, suitable for hybridoma

sterility

sterile-filtered

product line

BioReagent

form

liquid

technique(s)

cell culture | hybridoma: suitable
single cell analysis: suitable

impurities

endotoxin, tested

density

1.077 at 25 °C (relative density)(lit.)

storage temp.

2-8°C

Quality Level

400

Related Categories

Application

Histopaque®-1077 Hybri-Max™ has been used to isolate:
  • peripheral blood mononuclear cells (PBMCs) from heparinized blood by gradient centrifugation
  • immune cells (lymphocytes) from peripheral blood
  • PBMCs from blood

General description

Histopaque®-1077 is a solution comprising polysucrose and sodium diatrizoate, adjusted to a density of 1.077 +/- 0.001 g/cm3. This medium facilitates rapid recovery of viable lymphocytes from small volumes of whole blood. This technique is suitable for use in cell-mediated lympholysis and human lymphocyte antigen (HLA)2 typing. It may also be employed as the initial isolation step before the enumeration of T-, B-, and null lymphocytes. It is used to create a density medium for the purification of lymphocytes and other mononuclear cells.

Legal Information

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
RNBM0089
RNBL7498
RNBL3437
RNBL2041
RNBK6706

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Janet E Alexander et al.
The journals of gerontology. Series A, Biological sciences and medical sciences, 73(6), 720-728 (2017-11-11)
As in human populations, advances in nutrition and veterinary care have led to an increase in the lifespan of companion animals. Detrimental physiological changes occurring later in life must be understood before interventions can be made to slow or reduce
Carla Oseroff et al.
Proceedings of the National Academy of Sciences of the United States of America, 102(39), 13980-13985 (2005-09-21)
We have analyzed by ex vivo ELISPOT the anti-vaccinia cytotoxic T lymphocyte responses of peripheral blood mononuclear cells from humans vaccinated with Dryvax vaccine. More than 6,000 peptides from 258 putative vaccinia ORFs predicted to bind the common molecules of
Joshua E Messinger et al.
mBio, 10(4) (2019-07-04)
Deciphering the molecular pathogenesis of virally induced cancers is challenging due, in part, to the heterogeneity of both viral gene expression and host gene expression. Epstein-Barr virus (EBV) is a ubiquitous herpesvirus prevalent in B-cell lymphomas of immune-suppressed individuals. EBV
Siddharth Sharma et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 73(2), 160-167 (2008-01-15)
Coulter volume is far more accurate measure of cell volume than forward angle light scatter. In this report, we have used Coulter volume to determine the mean cell volume and diameter of normal human peripheral blood cells and hematopoietic progenitor
Mark P Yeager et al.
Anesthesia and analgesia, 127(2), 556-563 (2018-07-22)
Cortisol is a prototypical human stress hormone essential for life, yet the precise role of cortisol in the human stress response to injury or infection is still uncertain. Glucocorticoids (GCs) such as cortisol are widely understood to suppress inflammation and
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Protocols

Histopaque® Density Gradient Media Troubleshooting Guide

This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.

Histopaque® 密度梯度介质故障排除指南

本疑难排查指南针对使用 Histopaque 时观察到的最常见错误来源提出解决方案，但这并不代表本指南是全面无遗的。

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