H9037
Anti-HA−TRITC antibody, Mouse monoclonal
~1 mg/mL, clone HA-7, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-HA, Anti-HA, Anti-Influenza Hemagglutinin
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About This Item
Conjugate:
TRITC conjugate
Clone:
HA-7, monoclonal
Application:
IF (d)
Citations:
9
biological source
mouse
Quality Segment
conjugate
TRITC conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
HA-7, monoclonal
form
buffered aqueous solution
storage condition
protect from light
concentration
~1 mg/mL
technique(s)
direct immunofluorescence: 10-15 mg/mL using mammalian cells expressing HA-tagged fusion proteins
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
General description
Monoclonal Anti-HA is derived from the HA-7 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Monoclonal anti-HA recognizes the HA tag sequence on HA tagged fusion proteins by immunocytochemistry. The antibody reacts specifically with N- and C-terminal HA tagged fusion proteins.
Immunogen
synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH
Application
Monoclonal Anti-HA-TRITC antibody was used to test if DOT/ICM effector SDHA is necessary for virulence of Legionella pneumophila in Galleria mellonella and A/J mice.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunofluorescence (1 paper)
Biochem/physiol Actions
The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin 15 mM sodium azide as a preservative.
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Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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