The building block of chromatin is the nucleosome which is comprised of an octamer of core histones (H2A, H2B, H3, and H4) around which 147 bp of DNA are wrapped. Histones H3 and H4 are the predominant histones subjected to covalent modifications. Acetylation of histone H4 on Lys16 (H4-K16Ac) is a prevalent modification that plays a role in transcriptional activation by regulating higher-order chromatin structure and protein interactions.

acetyl-Histone H4 (Ac-Lys¹⁶) is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.

synthetic peptide corresponding to amino acids 9-18 (Ac-Lys16) of human histone H4, conjugated to KLH.

Anti-acetyl-Histone H4 (Ac-Lys16) antibody produced in rabbit is suitable for western blot at a concentration of 1-2μg/mL using HL60 cell lysates.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Immunohistochemistry (1 paper)

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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