H9166
Vascular Endothelial Growth Factor 165 human
≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
Synonym(s):
Endothelial Growth Factor, Vascular Growth Factor
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About This Item
Biological source:
human
Recombinant:
expressed in HEK 293 cells
Assay:
≥95% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
dimer 45 kDa (glycosylated)
Impurities:
≤1 EU/μg
Product Name
Vascular Endothelial Growth Factor 165 human, VEGF165, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture
biological source
human
Quality Segment
recombinant
expressed in HEK 293 cells
assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
≤5 ng/mL EC50
quality
endotoxin tested
mol wt
dimer 45 kDa (glycosylated)
packaging
pkg of 5x10 μg, pkg of 10 μg
manufacturer/tradename
HumanZyme
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/μg
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... VEGFA(7422)
General description
Recombinant human Vascular Endothelial Growth Factor 165 (VEGF165) is expressed in HEK 293 cells and supplied as a glycosylated homodimeric protein suitable for mammalian cell culture research. Glycosylation contributes to stability in cell growth media and other applications.
Application
VEGF165 has been used in endothelial cell culture research, including studies of cell proliferation, migration, and vascular‑related signaling in vitro.
Biochem/physiol Actions
Vascular Endothelial Growth Factor (VEGF165) is a mitogenic growth factor for endothelial cells and mediates cellular responses through VEGF receptor‑dependent signaling pathways in in vitro cell models. It is a potent angiogenic factor and mitogen that stimulates proliferation, migration and formation of endothelial cells.
Physical form
Lyophilized from a 0.2 μm filtered solution of 1X PBS
Analysis Note
The specific activity was determined by the dose-dependent stimulation of the proliferation of HUVEC cells (human umbilical vein endothelial cells).
Legal Information
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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