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H9658

Sigma-Aldrich

Monoclonal Anti-HA antibody produced in mouse

clone HA-7, ascites fluid

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Synonym(s):
Monoclonal Anti-HA, HA Antibody - Monoclonal Anti-HA antibody produced in mouse, Ha Antibody Sigma, Sigma Ha Antibody, Anti-HA, Anti-Influenza Hemagglutinin
MDL number:
MFCD00803873

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

HA-7, monoclonal

contains

15 mM sodium azide

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:20,000 using extracts from 293T (human embryonic kidney) cells expressing a recombinant HA-fusion protein

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

The gene encoding influenza virus hemagglutinin (HA) precursor is mapped on segment 4. It encodes a 76kDa surface glycoprotein which exists as trimers on the viral envelope.

Specificity

The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.

Immunogen

synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH

Application

Monoclonal Anti-HA antibody produced in mouse has been used in 293T cells for:
  • immunoprecipitation in human embryonic kidney 293 cells
  • immunoblotting in HeLa cells
  • immunopurification in human embryonic kidney 293 cells
  • immunostaining for electron microscopy in mouse embryonic fibroblasts
  • in immunocytochemistry of hippocampal slices
Monoclonal Anti-HA antibody produced in mouse has been used in:
  • Immunoblotting, to study the expression of fusion protein
  • Coimmunoprecipitation assay
  • Immunoblotting of lysate protein

Biochem/physiol Actions

Hemagglutinin (HA) is the surface glycoprotein of influenza virus. HA is crucial for virus entry and interaction with N-acetylneuraminic acid (Neu5Ac) in host cell surface. HA coordinates with neuraminidase (NA) and facilitates viral entry into host. HA exists as a trimer and each monomer has stalk transmembrane and globular regions. The globular region contains carbohydrate side chains crucial for infecting host.
Influenza virus hemagglutinin (HA) acts as a key immunogen in avian influenza virus (AIV) vaccines. Activated HA facilitates virus attachment to oligosaccharide receptors on host cell surface and is involved in penetration through fusion following conformational modifications in the endolysosomal compartment. 226 and 228 residues in hemagglutinin (HA) of the H3 subtype play a key role in host range restriction and receptor specificity. Influenza virus hemagglutinin (HA) is responsible for 1918 influenza pandemic.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

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    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the isotype and light chain for Monoclonal Anti-HA antibody clone HA-7 produced in mouse?

    Monoclonal Anti-HA antibody clone HA-7 is mouse IgG1, kappa light chain.

  6. What is the recommended dilution of Product H9658 - HA antibody for immunoprecipitation and for indirect immunostaining?

    H9658 - Monoclonal Anti-HA antibody produced in mouse is the ascites fluid. It is not purified. We only test this product in western blotting.  This product contains 15-55 mg/ml total protein with 4-7 mg/ml specific antibody. H3663 - Monoclonal Anti-HA antibody produced in mouse as the purified immunoglobulin. We test this product for immunoprecipitation using 0.5-2.0 μg and for immunocytochemistry using 1.0-2.0 μg/mL.These ranges would be a good starting concentration. In order to obtain best results in different techniques and preparations, we recommend determining optimal working dilution by titration test.

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Wei H, et al.
Neuropharmacology, 56(2), 399-404 (2009)
Influenza A virus hemagglutinin and neuraminidase act as novel motile machinery
Sakai T, et al.
Scientific Reports, 7, 45043-45043 (2017)
A single amino acid substitution in 1918 influenza virus hemagglutinin changes receptor binding specificity.
Glaser L, et al.
Journal of Virology, 79(17), 11533-11536 (2005)
Characterization of a novel influenza A virus hemagglutinin subtype (H16) obtained from black-headed gulls.
Fouchier R A M, et al.
Journal of Virology, 79(5), 2814-2822 (2005)
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Shi L, et al.
Molecular Oncology (2018)
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