Hepatocyte Growth Factor human

Recombinant human Hepatocyte Growth Factor (HGF) is expressed in NSO cells and supplied as a lyophilized protein suitable for mammalian cell culture research. HGF is a cytokine that signals through the c‑Met receptor and is widely studied in in vitro cell models.

Hepatocyte Growth Factor human has been used as a growth factor cocktail to suspend rat fibrogen/thrombin cells. It has also been used to study the tyrosine phosphorylation of MET (MET proto-oncogene, receptor tyrosine kinase) in myxofibrosarcoma cell line.

Hepatocyte Growth Factor (HGF) is a multifunctional growth factor that exhibits mitogenic and motogenic activity in vitro and mediates cellular responses via c‑Met receptor signaling pathways.[1] It′s major function is to induce in vitro hepatocytes proliferation. HGF is also essential for cell migration and angiogenesis. Itis associated with embryonic development and tissue homeostasis. It is known to mediate placental trophoblast invasion, which is essential for normal fetal growth.

Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of cell types including endothelial and epithelial cells, melanocytes, and keratinocytes. It also mediates epithelial morphogenesis. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 50 μg of bovine serum albumin per 1μg of cytokine.

The biological activity is measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells.

The NSO-expressed recombinant HGF is greater than 95% heterodimeric HGF. The insect cell line-expressed recombinant HGF is a mixture of single chain HGF (predominant) with some heterodimeric HGF. Single chain HGF and heterodimeric HGF are equally active in in vitro assays.