HSANGERG
Sanger Arrayed Whole Genome Lentiviral CRISPR Library
Human, Glycerol Format
Synonym(s):
Sanger CRISPR Library
Quality Level
packaging
pkg of 50 μL (96-well plate)
application(s)
CRISPR
shipped in
dry ice
storage temp.
−70°C
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General description
Application
Features and Benefits
- Vector: U6-gRNA/PGK-Puro-2A-BFP (gRNA only)
- Simplify the workflow with puromycin selection
- Illuminate CRISPR-expressing cells with BFP
- Flip the expression components into and out of the genome using transposase
Additional Features
- Better, not bigger: Two optimized clones per human gene reduces the time, cost, and scale of screening experiments
- Ready-to-screen: Clones are arrayed in a robotics-friendly 96-well format for high throughput screening; requires plasmid preparation to transfect cells or lentivirus preparation to transduce cells
- Collaborative: Real-time, library validation continues
For detailed information on the Sanger library, click here
Packaging
Physical form
Other Notes
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Legal Information
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Articles
Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.
全基因组功能缺失筛查是发现生物过程背后的基因和途径的有效方法。现在，每个基因都可通过两个优化的 gRNA 完全敲除。通过最大限度减少克隆数量，可确保尽可能特异性的筛选，同时控制时间和成本。
Protocols
Learn about Sanger Sequencing steps or the chain termination method and how DNA sequencing works and how to read Sanger Sequencing results accurately for your research.
了解桑格测序步骤或链终止法、DNA 测序的工作原理以及如何在研究中准确读取桑格测序结果。
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