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HT501128

Sigma-Aldrich

Formalin solution, neutral buffered, 10%

histological tissue fixative

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Synonym(s):
Formaldehyde, Methanal
MDL number:
PubChem Substance ID:
NACRES:
NA.47

biological source

synthetic

Quality Level

shelf life

Expiry date on the label.

concentration

10% formalin (approx. 4% formaldehyde)

color

colorless

pH

6.90-7.10

density

1.080 g/cm3

application(s)

hematology
histology

storage temp.

room temp

InChI

1S/CH2O/c1-2/h1H2

InChI key

WSFSSNUMVMOOMR-UHFFFAOYSA-N

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General description

10% Neutral buffered formalin (NBF) is the most commonly used fixative throughout the world for light microscopy and is a somewhat forgiving fixative. 10% NBF is well suited for large throughput laboratories, and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue. Formalin fixation is thought to form between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining.

Application

Formalin solution, neutral buffered, 10% has been used as a popular general purpose histological fixative to fix tissues.
Formalin solution, neutral buffered, 10% has been used in oil-red-o staining for the differentiation of 3T3-L1 preadipocyte cell line and mouse embryonic fibroblasts into adipocytes. It has been used in the histological analysis of rabbit′s eye and diabetic rat intestine.
Popular general purpose histological fixative.

Physical form

Neutral buffered solution

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Muta. 2 - Skin Sens. 1

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

185.0 °F

Flash Point(C)

85 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Jonathan C J Wei et al.
Scientific reports, 7(1), 15885-15885 (2017-11-23)
Emerging micro-scale medical devices are showing promise, whether in delivering drugs or extracting diagnostic biomarkers from skin. In progressing these devices through animal models towards clinical products, understanding the mechanical properties and skin tissue structure with which they interact will
Effects of the marine microalgae Isochrysis galbana and Nannochloropsis oculata in diabetic rats
Nu?o K, et al.
Journal of functional foods, 5(1) (2013)
Paul J Marchand et al.
Biomedical optics express, 8(7), 3343-3359 (2017-07-19)
We present a novel extended-focus optical coherence microscope (OCM) attaining 0.7 μm axial and 0.4 μm lateral resolution maintained over a depth of 40 μm, while preserving the advantages of Fourier domain OCM. Our system uses an ultra-broad spectrum from
Ablation of the transcriptional regulator Id1 enhances energy expenditure, increases insulin sensitivity, and protects against age and diet induced insulin resistance, and hepatosteatosis
Satyanarayana A, et al.
Faseb Journal, 26(1), 309-323 (2012)
Recapitulating spatiotemporal tumor heterogeneity in vitro through engineered breast cancer microtissues.
Mazio C, et al.
Acta Biomaterialia, 73, 236-249 (2018)

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