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HT501128

Formalin solution, neutral buffered, 10%

Name: Formalin solution, neutral buffered, 10%
Brand: SIGMA

histological tissue fixative

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Synonym(s):

Formaldehyde, Methanal

MDL number:

MFCD00003274

PubChem Substance ID:

24895887

NACRES:

NA.47

biological source

synthetic

shelf life

Expiry date on the label.

concentration

10% formalin (approx. 4% formaldehyde)

color

colorless

pH

6.90-7.10

density

1.080 g/cm³

application(s)

hematology
histology

storage temp.

room temp

InChI

1S/CH2O/c1-2/h1H2

InChI key

WSFSSNUMVMOOMR-UHFFFAOYSA-N

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Related Categories

Hematology Stains

Histology Stains

Tissue Diagnostics Universal Reagents

General description

10% Neutral buffered formalin (NBF) is the most commonly used fixative throughout the world for light microscopy and is a somewhat forgiving fixative. 10% NBF is well suited for large throughput laboratories, and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue. Formalin fixation is thought to form between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining.

Application

Formalin solution, neutral buffered, 10% has been used as a popular general purpose histological fixative to fix tissues.

Formalin solution, neutral buffered, 10% has been used in oil-red-o staining for the differentiation of 3T3-L1 preadipocyte cell line and mouse embryonic fibroblasts into adipocytes. It has been used in the histological analysis of rabbit′s eye and diabetic rat intestine.

Popular general purpose histological fixative.

Physical form

Neutral buffered solution

Pictograms

GHS07,GHS08

Signal Word

Danger

Hazard Statements

H302 + H332 - H317 - H341 - H350

Precautionary Statements

P202 - P280 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P308 + P313

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Muta. 2 - Skin Sens. 1

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

185.0 °F

Flash Point(C)

85 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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