Thermostable M-MLV Reverse Transcriptase

High Temp M-MLV (Moloney Murine Leukemia Virus) Reverse Transcriptase (HTRT) DNA polymerase uses DNA, single-stranded RNA, or an RNA:DNA hybrid (using a primer) to synthesize a complementary DNA strand . Wild type (WT) and traditional (MMLV-RT) are restricted to lower temperatures (up to 50℃) during cDNA synthesis. For many reverse transcription reactions, temperatures higher than 50℃ are beneficial. Higher temperatures reduce the chances of inhibition due to RNA secondary structures, while at the same time increasing primer binding specificity. These benefits lead to an increase in desired cDNA yield from the reverse transcription reaction. HTRT maintains its performance at high temperatures and outcompetes WT MMLV-RTs. This enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid.

HTRT reverse transcriptase is used for:

• preparation of cDNA libraries
• first strand cDNA synthesis for use in RT-PCR
• 2-step RT-PCR assay
• Quantitative realtime-polymerase chain reaction (RT-qPCR)